Immunogenicity of a Bivalent Non-Purified Recombinant Vaccine against Botulism in Cattle

Toxins
Clóvis MoreiraFabricio R Conceição

Abstract

Botulism is a potentially fatal intoxication caused by botulinum neurotoxins (BoNTs) produced mainly by Clostridium botulinum. Vaccination against BoNT serotypes C and D is the main procedure to control cattle botulism. Current vaccines contain formaldehyde-inactivated native BoNTs, which have a time-consuming production process and pose safety risks. The development of non-toxic recombinant vaccines has helped to overcome these limitations. This study aims to evaluate the humoral immune response generated by cattle immunized with non-purified recombinant fragments of BoNTs C and D. Cattle were vaccinated in a two-dose scheme with 100, 200 and 400 µg of each antigen, with serum sampling on days 0, 56, 120, and 180 after vaccination. Animals who received either 200 or 400 μg of both antigens induced titers higher than the minimum required by the Brazilian ministry of Agriculture, Livestock and Food Supply and achieved 100% (8/8) seroconversion rate. Animals vaccinated with commercial toxoid vaccine had only a 75% (6/8) seroconversion rate for both toxins. Animals that received doses containing 400 µg of recombinant protein were the only ones to maintain titers above the required level up until day 120 post-vaccination, and to ac...Continue Reading

References

May 13, 2004·Clinical and Diagnostic Laboratory Immunology·Hideyuki ArimitsuKeiji Oguma
Dec 12, 2007·Infection and Immunity·Michael R BaldwinJoseph T Barbieri
Mar 4, 2008·The Veterinary Journal·J G M HeldensTh P M Schetters
Oct 1, 2011·The Veterinary Journal·I MawhinneyB Strugnell
May 28, 2013·Toxicon : Official Journal of the International Society on Toxinology·Michel R Popoff, Philippe Bouvet
Jun 17, 2014·Anaerobe·G M S G MoreiraF C F Lobato
Mar 24, 2016·Scientific Reports·Gustavo Marçal Schmidt Garcia MoreiraFabricio Rochedo Conceição
May 29, 2016·Anaerobe·Clóvis MoreiraFabricio Rochedo Conceição
Mar 3, 2017·Japanese Journal of Infectious Diseases·Yasushi ToriiMotohide Takahashi
May 20, 2017·Genome Announcements·Cédric WoudstraPatrick Fach
Sep 5, 2017·Toxins·Grace Sundeen, Joseph T Barbieri
Jan 9, 2018·Vaccine·Amanda PrzedpelskiJoseph T Barbieri

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