PMID: 9188620Jul 1, 1997Paper

Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus

Journal of Virology
T D BrumeanuC A Bona

Abstract

A contiguously linked T-B synthetic viral epitope (110HA120-150HA159,T-B) was investigated for its potency in inducing humoral and cellular immune responses in vivo. The T-cell epitope 110HA120 corresponds to the site 1 hemagglutinin (HA) of the A/PR/8/34 (PR8) influenza virus and is recognized by CD4 T cells in association with I-Ed class II major histocompatibility complex molecules. The 150HA159 represents a major B-cell epitope of the HA protein. T-B dipeptide emulsified in Freund's complete adjuvant was able to induce strong antiviral antibody titers and a high frequency of specific T-cell precursors after a single inoculation in BALB/c mice. In contrast, immunization under identical conditions with equimolar mixtures of T and B peptides did not elicit antibody titers or a cellular immune response. As indicated by the isotypes of antiviral antibodies, the T-B dipeptide preferentially induced a Th1-like immune response. Challenge with T-B dipeptide, but not with T or B peptide alone, stimulated peptide-specific T memory cells in mice previously primed with PR8 virus or with T-B dipeptide. As a consequence, 71 and 57% of these mice, respectively, survived infection with two 100% lethal doses of PR8 virus. Our results suggest...Continue Reading

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Citations

May 3, 2007·Immunology·Caterina Hatzifoti, Andrew W Heath
Apr 28, 2000·The Journal of Peptide Research : Official Journal of the American Peptide Society·S Vichier-GuerreD Cantacuzène
Feb 13, 2001·Molecular Immunology·G S BuchanM A Baird

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