PMID: 8970972Dec 1, 1996Paper

Immunogenicity of an aphthovirus chimera of the glycoprotein of vesicular stomatitis virus

Journal of Virology
P R GrigeraR R Wagner

Abstract

An oligodeoxynucleotide coding for amino acids 139 through 149 of antigenic site A (ASA) of the VP1 capsid protein of the foot-and-mouth disease virus C3 serotype (FMDV C3) was inserted into three different in-frame sites of the vesicular stomatitis virus New Jersey serotype (VSV-NJ) glycoprotein (G) gene cDNA present in plasmid pKG97 under control of the bacteriophage T7 polymerase promoter. Transfection of these plasmids into CV1 cells coinfected with the T7 polymerase-expressing vaccinia virus recombinant vTF1-6,2 resulted in expression of chimeric proteins efficiently reactive with both anti-FMDV and anti-VSV G antibodies. However, in vitro translation of transcripts of these VSV-G/FMDV-ASA chimeric plasmids resulted in proteins that were recognized by anti-G serum but not by anti-FMDV serum, indicating a requirement for in vivo conformation to expose the ASA antigenic determinant. Insertion of DNA coding for a dimer of the ASA unidecapeptide between the VSV-NJ G gene region coding for amino acids 160 and 161 gave rise to a chimeric ASA-dimer protein designated GF2d, which reacted twice as strongly with anti-FMDV antibody as did chimeric proteins in which the ASA monomer was inserted in the same position or two other G-gene...Continue Reading

References

Jan 1, 1978·Virology·C D DenoyaJ L La Torre
Apr 1, 1992·Journal of Virology·K C McCulloughU Kihm
Nov 1, 1990·Nature·B MossT R Fuerst
Mar 16, 1989·Nature·S C Harrison
Apr 1, 1982·The Journal of General Virology·K StrohmaierK H Adam
Jul 1, 1995·Vaccine·R H MeloenJ P Langeveld

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Citations

Jun 24, 2003·Infection and Immunity·Alejandra V E CapozzoMarina S Palermo

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