Immunogenicity of Brucella abortus salt-extractable proteins

Veterinary Microbiology
L B TabatabaiJ M Patterson

Abstract

The immunogenic properties of salt-extractable proteins and chromatographic fractions thereof from Brucella abortus were evaluated in lemmings (Dicrostonyx rubricatus). The efficacy of the Brucella proteins as immunogens was determined after challenge with virulent B. abortus strain 2308 and was based on protection against clinical signs and gross lesions of brucellosis, as well as on numbers of viable Brucella in the spleen. Vaccination of lemmings with as little as 0.1 microgram of salt-extractable proteins (CSP) suppressed splenic infection, resulting in reduced numbers of viable organisms per spleen of 5-6 logs compared to non-vaccinated controls. Protein fractions separated by column chromatography were generally effective in reducing splenic infection, and contained proteins with molecular weights of 30,000, 20,000 and 12,000. Vaccines containing chemically modified dodecanoyl-CSP offered no additional advantage over unmodified CSP vaccines.

References

Nov 1, 1979·Infection and Immunity·L B TabatabaiA E Ritchie
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Jan 1, 1987·Annales De L'Institut Pasteur. Microbiology·M Plommet
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Jul 1, 1983·Infection and Immunity·M Plommet, A M Plommet
Aug 1, 1984·Journal of Clinical Microbiology·L B Tabatabai, B L Deyoe

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Citations

Dec 1, 1988·Veterinary Microbiology·B J BrickerJ E Mayfield
Sep 8, 2009·The Veterinary Journal·Alcina V Carvalho NetaRenato L Santos
Jan 16, 1990·Biochemistry·B L BeckJ E Mayfield
May 1, 1995·Clinical and Diagnostic Laboratory Immunology·M C Moreno-LafontR López-Santiago
Sep 1, 1990·Infection and Immunity·B J BrickerJ E Mayfield

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