PMID: 8958139Jan 1, 1997Paper

Immunogenicity of DNA damaged by reactive oxygen species--implications for anti-DNA antibodies in lupus

Free Radical Biology & Medicine
M S CookeJ Lunec

Abstract

Reactive oxygen species (ROS) are implicated in the inflammatory, autoimmune, connective tissue disease, systemic lupus erythematosus (SLE), particularly in respect of processes leading to the formation of pathological anti-DNA antibodies. Exposure to ROS increases the antigenicity of DNA for SLE antibodies, but data on the immunogenicity of ROS-DNA are not conclusive. In this study, we have examined the immunogenicity in rabbits, of DNA modified by three hydroxyl radical generating systems. Additionally, we investigated the antigenicity of UVA, UVB, and UVC irradiated DNA for lupus anti-DNA antibodies. Modification of DNA by both ROS and far UV dramatically increased its immunogenicity; the Fe2+ and H2O2 system resulted in antibodies that recognized both native and modified DNA. In our ELISA system, none of the UV antigens showed any antigenicity above native DNA for SLE sera. The data suggested that different profiles of antigenicity and immunogenicity arise dependent on the method of ROS production, but also that ROS-DNA may be a factor in antigen-driven immune complex formation in SLE.

References

Oct 1, 1992·Immunological Investigations·J AraR Ali
Jul 30, 1992·Journal of Photochemistry and Photobiology. B, Biology·R A LarsonR W Tuveson
Jan 1, 1992·Pharmacology & Therapeutics·K Frenkel
Jan 1, 1991·Free Radical Biology & Medicine·M Dizdaroglu
Mar 1, 1991·Archives of Biochemistry and Biophysics·M DizdarogluE Gajewski
Jan 1, 1991·Molecular Aspects of Medicine·S BlountJ Lunec
Mar 13, 1989·FEBS Letters·S BlountJ Lunec
Jun 1, 1989·Photochemistry and Photobiology·D L Mitchell, R S Nairn
Sep 1, 1988·The Journal of Clinical Investigation·I SchraufstätterC G Cochrane
Jul 1, 1986·Analytical Biochemistry·M Dizdaroglu, D S Bergtold
Apr 1, 1987·Photochemistry and Photobiology·G EggsetH Krokan
Mar 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·E M Tan, R B Stoughton
Apr 1, 1983·Medical Hypotheses·S S Hendler, M F McCarty
Aug 1, 1983·Mutation Research·S A Leadon, P C Hanawalt
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·I Emerit, A M Michelson
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
Jun 1, 1995·Free Radical Biology & Medicine·T H ZastawnyG Rao
Feb 1, 1995·Free Radical Biology & Medicine·S J Stohs, D Bagchi
Sep 1, 1993·Annals of the Rheumatic Diseases·S BashirP G Winyard
May 1, 1993·Free Radical Biology & Medicine·K FrenkelE Taioli
Jan 1, 1993·Environmental and Molecular Mutagenesis·F E AhmedN Setlow

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Citations

May 14, 1999·Arthritis and Rheumatism·Z AmouraS Koutouzov
Aug 14, 2001·Journal of Cellular Biochemistry·R ChiaramonteG V Sherbet
Jul 30, 2009·Rheumatology International·Rosa Weiss TellesEmilia Inoue Sato
Jun 22, 2011·Rheumatology International·Zaixing YangRenqian Zhong
May 7, 2009·Apoptosis : an International Journal on Programmed Cell Death·Young-Ok SonJeong-Chae Lee
Jun 24, 2010·Journal of Clinical Immunology·Ying-Ju LinFuu-Jen Tsai
Sep 3, 2004·Mutation Research·Mark D EvansMarcus S Cooke
Jun 12, 2003·Journal of Immunological Methods·Aikaterini KarakoulaMarcus S Cooke
Dec 19, 2002·Free Radical Biology & Medicine·Marcus S CookeMark D Evans
Jan 11, 2003·Free Radical Biology & Medicine·Marcus S CookeJoseph Lunec
Jun 25, 1999·Free Radical Biology & Medicine·N MistryJ Lunec
May 2, 2002·Bioorganic & Medicinal Chemistry·Hiroshi MarusawaTakahiro Tezuka
Oct 26, 2006·Nature Reviews. Immunology·Ann Marshak-Rothstein
Sep 29, 2001·Kidney International·K YohS Takahashi
Mar 19, 2003·Archives of Environmental Health·Etsuko MaeshimaSusumu Yukawa
Apr 1, 2005·Journal of Toxicology and Environmental Health. Part a·Sungwook ChaeSam Sik Kang
Jun 15, 2007·Journal of Toxicology and Environmental Health. Part a·Rui ZhangJin Won Hyun
Jul 27, 2007·Journal of Toxicology and Environmental Health. Part a·Kyoung Ah KangJin Won Hyun
Oct 31, 1998·Immunological Reviews·J F BachP M van Endert
Sep 2, 2006·Biological & Pharmaceutical Bulletin·Kyoung Ah KangJin Won Hyun
May 26, 2001·Biochemical and Biophysical Research Communications·M S CookeJ Lunec
Oct 7, 2004·Biological & Pharmaceutical Bulletin·Sungwook ChaeSam Sik Kang
Jun 1, 2010·Journal of Cellular Biochemistry·Shao-Hsuan KaoBor-Show Tzang
Oct 13, 2006·Scandinavian Journal of Immunology·F KhanR Ali
Aug 12, 2010·International Journal of Radiation Biology·Ergüder B ImgeIlker Durak
Oct 24, 2013·BioMed Research International·Wahid Ali Khan, Mohd Wajid Ali Khan
Sep 6, 2008·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Hsing-Hui LiMing-Shi Chang
Jul 21, 2004·Rheumatic Diseases Clinics of North America·Sophie KoutouzovZahir Amoura
Jan 17, 2003·Lupus·J W C DiekerJ H M Berden
Nov 11, 1999·FEBS Letters·J AhmadJ Lunec

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