Immunogenicity of meningococcal PBP2 during natural infection and protective activity of anti-PBP2 antibodies against meningococcal bacteraemia in mice

The Journal of Antimicrobial Chemotherapy
Maria Leticia ZarantonelliMuhamed-Kheir Taha

Abstract

To evaluate the immunogenicity of the meningococcal penicillin-binding protein 2 (PBP2) and its potential as a vaccine candidate. The immunogenicity of meningococcal PBP2 was investigated using acute and convalescent sera from patients who recovered from meningococcal disease. Sera were tested against purified recombinant PBP2s corresponding to meningococcal isolates of different genetic lineages, of different serogroups and with various susceptibility levels to penicillin G. Mice were vaccinated with recombinant PBP2 and challenged with Neisseria meningitidis. A purified anti-PBP2 rabbit IgG was also used for passive protection experiments in mice. Convalescent patients' sera recognized PBP2s from different strains, showing that this protein is immunogenic in meningococcal disease. Vaccination with purified recombinant PBP2 and purified anti-PBP2 rabbit IgG antibody conferred protection against experimental meningococcaemia in mice. These data argue for considering meningococcal PBP2 as a vaccine candidate.

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Citations

Apr 28, 2012·The Journal of Infectious Diseases·Mohd Akhlakur RahmanYasuko Rikihisa
Sep 26, 2007·Infection and Immunity·Maria-Leticia ZarantonelliMuhamed-Kheir Taha
Nov 18, 2011·Journal of Nanobiotechnology·Ives B MattosMarcelo Lancellotti
Dec 26, 2009·Comparative Immunology, Microbiology and Infectious Diseases·Julia W PridgeonPhillip H Klesius
Jul 17, 2013·Vaccine·Valérie DelbosUNKNOWN B14 STOP study group
Apr 4, 2017·Pathogens and Disease·Myron Christodoulides, John Heckels
Oct 2, 2019·Nature Biotechnology·Karissa L CrossMircea Podar
Jan 21, 2021·International Journal of Molecular Sciences·Abid Ali KhanHans-Peter Deigner

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