Immunogenicity of plant-produced porcine circovirus-like particles in mice

Plant Biotechnology Journal
Cornelius J GunterInga I Hitzeroth

Abstract

Porcine circovirus type 2 (PCV-2) is the main causative agent associated with a group of diseases collectively known as porcine circovirus-associated disease (PCAD). There is a significant economic strain on the global swine industry due to PCAD and the production of commercial PCV-2 vaccines is expensive. Plant expression systems are increasingly regarded as a viable technology to produce recombinant proteins for use as pharmaceutical agents and vaccines. However, successful production and purification of PCV-2 capsid protein (CP) from plants is an essential first step towards the goal of a plant-produced PCV-2 vaccine candidate. In this study, the PCV-2 CP was transiently expressed in Nicotiana benthamiana plants via agroinfiltration and PCV-2 CP was successfully purified using sucrose gradient ultracentrifugation. The CP self-assembled into virus-like particles (VLPs) resembling native virions and up to 6.5 mg of VLPs could be purified from 1 kg of leaf wet weight. Mice immunized with the plant-produced PCV-2 VLPs elicited specific antibody responses to PCV-2 CP. This is the first report describing the expression of PCV-2 CP in plants, the confirmation of its assembly into VLPs and the demonstration of their use to elicit a ...Continue Reading

References

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Citations

Sep 6, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Edward P Rybicki
Jul 9, 2020·Plants·Balamurugan ShanmugarajWaranyoo Phoolcharoen
Oct 17, 2020·Frontiers in Veterinary Science·Naila ShahidAbdul Qayyum Rao
May 1, 2021·Vaccines·Jennifer Schwestka, Eva Stoger

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Datasets Mentioned

BETA
KJ128269

Methods Mentioned

BETA
density gradient centrifugation
electron microscopy
transmission electron microscopy
ELISA
protein folding
PCR

Software Mentioned

Image Studio Lite
numpy
Python
Byonic Software Metrics
matplotlib
Image [UNK] Lite
Excel Office ProPlus

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