Immunogenicity of subcellular fractions of Brucella abortus: measurement by in vitro lymphocyte proliferative responses

Veterinary Immunology and Immunopathology
R SmithA M Wu

Abstract

Five groups of heifers were immunized with various subcellular fractions of Brucella abortus and tested for their responsiveness in lymphocyte proliferative responses in vitro. The five subcellular fractions used as immunogens were: (1) a mixture of recombinant outer membrane proteins fused to Escherichia coli beta-galactosidase, (2) a mixture of outer membrane proteins BaomI, BaomIIB1, and BaomIII1, (3) a mixture of outer membrane proteins 7.5 kDa and 8.8 kDa, (4) a complex of smooth lipopolysaccharide and proteins, and (5) a complex of outer membranes and peptidoglycan (OM-PG complex) from a rough strain. All immunogens were emulsified in adjuvant and administered twice at a 61-day interval. Two other groups of cows were included; one immunized with strain 19 and the other with adjuvant only. Strain 19 and the rough OM-PG complex induced responsiveness in lymphocyte proliferation assays in a high percentage of immunized cows. The smooth lipopolysaccharide-protein complex induced responsiveness in fewer cows. The lowest frequencies of responding cows were found in groups that received either recombinant proteins or purified protein mixtures. Based on these results, we concluded: (1) cellular immunity, as measured by in vitro l...Continue Reading

References

Dec 1, 1986·Journal of Reproductive Immunology·A J WinterW L Castleman
Feb 1, 1985·Infection and Immunity·C L Baldwin, A J Winter
Aug 1, 1985·Veterinary Immunology and Immunopathology·C L BaldwinA J Winter
Jan 1, 1982·Veterinary Immunology and Immunopathology·F M Collins, S G Campbell
Jul 1, 1983·Veterinary Immunology and Immunopathology·P J HolmanG G Wagner
Nov 1, 1981·Reviews of Infectious Diseases·H Hahn, S H Kaufmann

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Citations

Nov 5, 2002·Veterinary Microbiology·John H Wyckoff
Jun 7, 2015·Vaccine·Elaine M S DornelesAndrey P Lage
Oct 2, 2007·Veterinary Immunology and Immunopathology·John H Wyckoff, Richard D Potts

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