Immunogenicity of superoxide radical modified-DNA: studies on induced antibodies and SLE anti-DNA autoantibodies

Life Sciences
Gulam Waris, Khurshid Alam

Abstract

Superoxide anion radical (SAR) is formed in almost all aerobic cells and it is the most abundant species generated by several enzymatic and non-enzymatic pathways in mammalian tissues, leading to unfavorable alteration of biomolecules including DNA. The SAR-modified macromolecules have been implicated in several disease states including disorders of inflammation. The SAR-induced damage to DNA showed hyperchromicity, single strand breaks, decrease in melting temperature, and modification of bases. Superoxide modified-DNA in rabbits elicited high titer antibodies and showed diverse antigens binding characteristics. The induced antibodies recognized native DNA and other nucleic acid polymers. Anti-DNA IgG from SLE sera, purified on Protein-A-Sepharose matrix, exhibited increased recognition of superoxide anion radical modified-DNA than native DNA in competitive immunoassay. The visual formation of immune complex between induced antibodies and native DNA, and between SLE anti-DNA IgG and superoxide modified-DNA, is a clear indication of property sharing between SLE autoantibodies and experimentally induced antibodies against superoxide modified-DNA.

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Citations

Sep 4, 2010·Journal of Clinical Immunology·Wahid Ali KhanAbdullah S Assiri
Sep 19, 2006·Biochemistry. Biokhimii︠a︡·Fozia KhanRashid Ali
May 13, 2006·Journal of Carcinogenesis·Gulam Waris, Haseeb Ahsan
Aug 25, 2007·Cellular Immunology·Khurshid AlamSuraya Jabeen
Oct 13, 2006·Scandinavian Journal of Immunology·F KhanR Ali
Apr 6, 2006·Journal of Biochemistry and Molecular Biology·Fozia Khan, Rashid Ali
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Dec 10, 2015·Journal of Biomolecular Structure & Dynamics·N I AkberovaR I Litvinov
Jul 17, 2007·Archives of Biochemistry and Biophysics·Wahid Ali Khan Moinuddin
Feb 4, 2014·The Journal of Rheumatology·Gustavo Martelli PalominoEduardo A Donadi
Dec 15, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·T I AyudhyaN N Dingra
Jun 11, 2008·Molecular and Cellular Biochemistry·Wahid Ali Khan Moinuddin
Aug 18, 2018·Journal of Immunology Research·Martha Paola Mireles-CanalesCésar Pacheco-Tena

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