Immunoglobulin and T-cell receptor gene rearrangements and its significance in the diagnosis of non-Hodgkin's lymphomas
Zhonghua zhong liu za zhi [Chinese journal of oncology]
J Z Zhang
Twenty-seven cases of non-Hodgkin's lymphoma (NHL) were studied by Southern blot to analyse gene rearrangements of immunoglobulin heavy chain (IgH), kappa chain (Igk) and T-cell receptor beta (T beta). Genomic DNA extraction from tissues was modified by increasing concentration of proteinase k and SDS in the extraction buffer and prolongation of incubation time. The results showed that 26 of 27 cases (96%) presented gene rearrangements of immunoglobulin and T-cell receptor of NHLs with T-cell phenotype, 11 of 13 cases showed T beta gene rearrangement. All the B-cell lymphomas analysed displayed IgH and Igk gene rearrangements. Reactive lymphoid hyperplasia demonstrated germline configuration of the above mentioned genes. These results suggest that gene rearrangement analysis may considerably help in determining the clonality and lineage of lymphomas, and distinguish benign reactive lymphoia hyperplasia from malignant lymphoma.
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