PMID: 2510157Oct 1, 1989Paper

Immunoglobulin double-isotype expression by trans-mRNA in a human immunoglobulin transgenic mouse

Proceedings of the National Academy of Sciences of the United States of America
A ShimizuT Honjo

Abstract

We have studied immunoglobulin double-isotype expression in a transgenic mouse (TG.SA) in which expression of the endogenous immunoglobulin heavy chain locus is almost completely excluded by a nonallelic rearranged human mu transgene. By flow-cytometric analyses, we have shown that a small, but significant, portion (about 4%) of transgenic spleen cells expresses human mu (transgene) and mouse gamma (endogenous) chains when cultured in vitro with bacterial lipopolysaccharide and interleukin 4. By using amplification of cDNA by the polymerase chain reaction, followed by cloning and sequencing of the amplified cDNA fragment, we have demonstrated expression of trans-mRNA consisting of the transgenic variable and endogenous constant (gamma 1) region sequences. Such trans-mRNA could be produced by either switch recombination or trans-splicing between the transgene and endogenous sterile gamma 1-gene transcripts. These results indicate that trans-splicing might be a possible mechanism for the immunoglobulin double-isotype expression in normal B lymphocytes that have not rearranged the second expressed constant region gene.

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Citations

Nov 8, 2003·Journal of Immunological Methods·Jacob SchlesingerGideon Gross
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·T KawasakiN Ichikawa
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T E CostaM C Nussenzweig
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L XuP Rothman
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·J P Bruzik, T Maniatis
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May 1, 1996·Nucleic Acids Research·Z Pasman, M A Garcia-Blanco
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Sep 13, 2005·Developmental and Comparative Immunology·Eva BengténMelanie Wilson
Sep 27, 2005·Memórias do Instituto Oswaldo Cruz·Mario Gustavo Mayer, Lucile Maria Floeter-Winter
Mar 30, 1999·Nature Biotechnology·M PuttarajuL G Mitchell
Jul 31, 2004·Blood·Francesco ForconiFreda K Stevenson
Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva BengténMelanie Wilson
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole D'AvirroErik Selsing
Jan 22, 2005·Immunological Reviews·Svend Petersen-Mahrt

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