PMID: 6795715Jan 1, 1981

Immunoglobulin expression in human lymphoblastoid cell lines with early B cell features

Scandinavian Journal of Immunology
P Guglielmi, J L Preud'homme


Immunoglobulin expression was studied by direct immunofluorescence and by biosynthesis experiments in two human cell lines Raji and T 5.1. The basic phenotype of these cells was close to that of pre-B cells: large cells with intracytoplasmic IgM with a predominance of mu chains over light chains and no detectable surface immunoglobulins. The apparent molecular weight of heavy and light chains was abnormally large. Immunoglobulins were secreted at a low rate as pentameric IgM in the T 5.1 line and as subunits and free light chains in the Raji line. Spontaneous variations of this phenotype were observed: the cultured cells acquired mu and lambda chains, then additionally delta chains while they progressively lost detectable cytoplasmic mu chains, thus leading to a mature B cell phenotype. Subsequently, the cells had no detectable surface and cytoplasmic immunoglobulins and then they displayed a pre-B cell phenotype again. Attempts to induce further maturation using various potential inducers were unsuccessful.


Jan 1, 1976·Scandinavian Journal of Immunology·R J WinchesterT Hoffman
Dec 1, 1977·European Journal of Immunology·J L Preud'hommeM Seligmann
Feb 1, 1979·International Journal of Cancer. Journal International Du Cancer·R HurwitzJ Kersey
Nov 1, 1978·The Journal of Experimental Medicine·A R HaywardM D Cooper
Dec 1, 1978·European Journal of Immunology·Y Bergman, J Haimovich
Nov 16, 1978·Nature·M G ToveyJ Begon-Lours
Apr 20, 1978·The New England Journal of Medicine·L B VoglerM D Cooper
Jan 1, 1979·Scandinavian Journal of Immunology·J L Preud'hommeM Seligmann
Jan 1, 1977·International Journal of Cancer. Journal International Du Cancer·G KleinG Clements
Nov 1, 1977·European Journal of Immunology·D KillanderH A Fabricius
Jan 1, 1975·International Archives of Allergy and Applied Immunology·S Gupta, M H Grieco
Jan 1, 1975·European Journal of Immunology·M FerrariniM L Durante
Aug 1, 1975·European Journal of Immunology·L MorettaM C Mingari
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·K SatoJ W Littlefield
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C FriendT Sato
May 1, 1965·Journal of Clinical Pathology·J V PULVERTAFT


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Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J A HatzfeldJ Maigne
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K S LandrethS M Fu
Dec 20, 2002·Thyroid : Official Journal of the American Thyroid Association·Deng-Huang Su, Tien-Shang Huang
Jan 1, 1982·British Journal of Haematology·P GuglielmiM T Daniel
Jun 1, 2005·Virology·William B KlimstraHans W Heidner
Aug 1, 1983·Scandinavian Journal of Immunology·E MihaescoC Mihaesco
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May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bassam M BadranKaren E Willard-Gallo
May 10, 2019·International Journal of Laboratory Hematology·Marie C Béné

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