PMID: 6795715Jan 1, 1981

Immunoglobulin expression in human lymphoblastoid cell lines with early B cell features

Scandinavian Journal of Immunology
P Guglielmi, J L Preud'homme

Abstract

Immunoglobulin expression was studied by direct immunofluorescence and by biosynthesis experiments in two human cell lines Raji and T 5.1. The basic phenotype of these cells was close to that of pre-B cells: large cells with intracytoplasmic IgM with a predominance of mu chains over light chains and no detectable surface immunoglobulins. The apparent molecular weight of heavy and light chains was abnormally large. Immunoglobulins were secreted at a low rate as pentameric IgM in the T 5.1 line and as subunits and free light chains in the Raji line. Spontaneous variations of this phenotype were observed: the cultured cells acquired mu and lambda chains, then additionally delta chains while they progressively lost detectable cytoplasmic mu chains, thus leading to a mature B cell phenotype. Subsequently, the cells had no detectable surface and cytoplasmic immunoglobulins and then they displayed a pre-B cell phenotype again. Attempts to induce further maturation using various potential inducers were unsuccessful.

References

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Citations

Jul 1, 1982·Journal of Clinical Immunology·P GuglielmiM Seligmann
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J A HatzfeldJ Maigne
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K S LandrethS M Fu
Dec 20, 2002·Thyroid : Official Journal of the American Thyroid Association·Deng-Huang Su, Tien-Shang Huang
Jan 1, 1982·British Journal of Haematology·P GuglielmiM T Daniel
Jun 1, 2005·Virology·William B KlimstraHans W Heidner
Aug 1, 1983·Scandinavian Journal of Immunology·E MihaescoC Mihaesco
Dec 2, 2014·Biomolecular Concepts·Atsuka MatsuiKentaro Semba
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bassam M BadranKaren E Willard-Gallo
May 10, 2019·International Journal of Laboratory Hematology·Marie C Béné

Related Concepts

B-Lymphocytes
SDS-PAGE
Immunofluorescence Assay
IgM2
Immunoglobulins
Gamma-2b-Immunoglobulin Heavy Chain
Lambda-x Immunoglobulin
Lymphocyte Activation
Receptors, Antigen, B-Cell

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