PMID: 6402777Feb 1, 1983Paper

Immunoglobulin gene expression in transformed lymphoid cells

Proceedings of the National Academy of Sciences of the United States of America
V T OiP Berg

Abstract

Myeloma, hybridoma, and thymoma cell lines have been successfully transfected for the Escherichia coli xanthine-guanine phosphoribosyltransferase gene (gpt) by using the plasmid vector pSV2-gpt. The transformed cells synthesize the bacterial enzyme 5-phospho-alpha-D-ribose-1-diphosphate:xanthine phosphoribosyltransferase (XGPRT; EC 2.4.2.22) and have been maintained in selective medium for over 4 months. Lymphoid cell lines expressing a K immunoglobulin light chain were obtained by transfecting cells with pSV2-gpt containing a rearranged K light chain genomic segment from the S107 myeloma cell line. The S107 light chain is synthesized in gpt-transformed J558L myeloma cells and is identical to the light chain synthesized by the S107 myeloma cell line, as judged by immunoprecipitation and two-dimensional gel electrophoresis. Furthermore, this light chain is synthesized and secreted as part of an intact antibody molecule by transformed hybridoma cells that normally secrete an IgGl (gamma, K) antibody molecule. No light chain synthesis was detected in a similarly transformed rat myeloma or a mouse thymoma line.

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Citations

Mar 1, 1988·Somatic Cell and Molecular Genetics·D F SchorderetS M Gartler
Mar 1, 1984·Somatic Cell and Molecular Genetics·F C RieraH S Kaplan
Jun 1, 1994·Molecular Biotechnology·S J ForsterA A Hamilton
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Jan 23, 2002·Immunology Letters·Alina HeyfetsNurit Hollander
Sep 2, 1998·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·S K Vaswani, R G Hamilton
Jul 13, 2011·Journal of Proteome Research·Jose Manuel Garcia-ManteigaRoberto Sitia
Apr 1, 1992·Bio/technology·H UedaH Nishimura
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A OchiN Hozumi
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A Seiler-TuynsB M Paterson
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Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K YoungA Bank

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