Immunoglobulin immobilized liposomal constructs for transmucosal vaccination through nasal route
Abstract
The aim of the present investigation was to evaluate the prospective of surface-engineered vesicular carriers for mucosal immunization via the nasal route. IgG antibody was immobilized on the surface of hepatitis B surface antigen (HBsAg) antigen-loaded liposomes. The developed formulations were characterized on the basis of physicochemical parameters, such as morphology, particle size, polydispersity index, entrapment efficiency, and zeta potential. Liposomal formulations were then evaluated for in-process antigen stability and storage stability. In vivo studies were conducted to visualize targeting potential, localization pattern, and immunogenicity. In addition, immune response was compared with alum-HBsAg vaccine injected intramuscularly. The serum anti-HBsAg titer, obtained from the postnasal administration of IgG-coupled liposomes, was significantly higher than plain liposomes. Moreover, IgG-coupled liposomes generated both humoral (i.e., systemic and mucosal) and cellular immune responses upon nasal administration, while the alum-adsorbed antigen displayed neither cellular (cytokine level) nor mucosal (IgA) response. The formulation also displayed enhanced transmucosal transport, improved in vitro stability, and effectiv...Continue Reading
References
Oral treatment of haemophilia A by gastrointestinal absorption of factor VIII entrapped in liposomes
Mucoadhesive liposomes for intranasal immunization with an avian influenza virus vaccine in chickens
Citations
Severe refractory thrombocytopenia, dengue hemorrhagic fever, and intravenous anti-D immune globulin
Related Concepts
Related Feeds
Avian Influenza: Innate Immune Adjuvant
Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.
Avian Influenza: Innate Immune Adjuvant (ASM)
Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.