PMID: 2508061Sep 25, 1989Paper

Immunoglobulin kappa light chain gene promoter and enhancer are not responsible for B-cell restricted gene rearrangement

Nucleic Acids Research
M GoodhardtF Rougeon

Abstract

We have produced transgenic mice which synthesize chimeric mouse-rabbit immunoglobulin (Ig) kappa light chains following in vivo recombination of an injected unrearranged kappa gene. The exogenous gene construct contained a mouse germ-line kappa variable (V kappa) gene segment, the mouse germ-line joining (J kappa) locus including the enhancer, and the rabbit b9 constant (C kappa) region. A high level of V-J recombination of the kappa transgene was observed in spleen of the transgenic mice. Surprisingly, a particularly high degree of variability in the exact site of recombination and the presence of non germ-line encoded nucleotides (N-regions) were found at the V-J junction of the rearranged kappa transgene. Furthermore, unlike endogenous kappa genes, rearrangement of the exogenous gene occurred in T-cells of the transgenic mice. These results show that additional sequences, other than the heptamer-nonamer signal sequences and the promoter and enhancer elements, are required to obtain stage- and lineage- specific regulation of Ig kappa light chain gene rearrangement in vivo.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1986·Annual Review of Immunology·G D Yancopoulos, F W Alt
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D Weaver, D Baltimore
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M GoodhardtF Rougeon
Oct 1, 1987·Genes & Development·M R LieberM Gellert
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M NishiT Honjo
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F W Alt, D Baltimore
Apr 14, 1983·Nature·S Tonegawa

❮ Previous
Next ❯

Citations

Sep 15, 1991·Experientia·S Rusconi
Oct 11, 1990·Nucleic Acids Research·K B MeyerM S Neuberger
Jan 1, 1995·International Reviews of Immunology·N Lonberg, D Huszar
Jul 10, 2004·Immunological Reviews·Mark S Schlissel
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I CoquilleauM Goodhardt
Feb 25, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel C McDevitBarbara S Nikolajczyk
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S LiW T Garrard
Jun 1, 1996·Molecular and Cellular Biology·M C RoqueV C Blasquez

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.