PMID: 4883841Dec 1, 1968Paper

Immunoglobulin lambda-chains. The complete amino acid sequence of a Bence-Jones protein

The Biochemical Journal
C MilsteinJ M Jarvis

Abstract

The total amino acid sequence of a lambda Bence-Jones protein has been established. The protein contains 211 residues, which include two methionine residues. Splitting with cyanogen bromide gave three fragments, the largest of which included the C-terminal half, which is common to other Bence-Jones proteins of the same type. The peptides obtained by tryptic, chymotryptic and peptic digestion were isolated and purified by paper-electrophoretic and chromatographic techniques. Reduction followed by carboxymethylation of the cysteine residues with radioactive iodoacetate was found to be a powerful tool in the isolation of some insoluble peptides. Unusual features of the molecule are the fact that it contains six cysteine residues and not five as observed in both kappa and lambda Bence-Jones proteins studied previously, and its size, which seems two residues smaller than the smallest Bence-Jones protein studied hitherto. The similarities and differences between this and other Bence-Jones proteins are discussed.

Citations

Jan 1, 1976·Scandinavian Journal of Immunology·P WestermarkG Husby
Jun 1, 1990·European Journal of Immunology·P ChuchanaM P Lefranc
Apr 1, 1970·European Journal of Biochemistry·F W Tischendorf, M M Tischendorf
Aug 23, 1969·Nature·E M Press, N M Hogg
Dec 31, 2019·BioMed Research International·Monika Gudowska-Sawczuk, Barbara Mroczko
Jan 1, 1969·International Journal of Protein Research·B S Harrap
Jul 1, 1981·Scandinavian Journal of Immunology·J B NatvigT Michaelsen
Sep 1, 1969·Die Naturwissenschaften·F W Putnam, H Köhler
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Jan 1, 1970·Progress in Biophysics and Molecular Biology·C Milstein, J R Pink
Aug 29, 1997·Clinica Chimica Acta; International Journal of Clinical Chemistry·K Fujita
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Jan 1, 1980·The International Journal of Biochemistry·D M Kirschenbaum
Dec 19, 1973·Biochemical and Biophysical Research Communications·C Tonnelle

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