Immunoglobulin-like transcript 3-Fc suppresses T-cell responses to allogeneic human islet transplants in hu-NOD/SCID mice.

Diabetes
George VladN Suciu-Foca

Abstract

The aim of our study was to explore the immunomodulatory activity of soluble immunoglobulin (Ig)-like transcript (ILT) 3-Fc in pancreatic islet transplantation and to determine its mechanism of action. NOD/SCID mice in which diabetes was induced by streptozotocin injection were transplanted with human pancreatic islet cells. Mice in which the transplant restored euglycemia were humanized with allogeneic peripheral blood mononuclear cells and treated with ILT3-Fc or control human IgG or left untreated. The blood glucose level was monitored twice a week, and rejection was diagnosed after two consecutive readings >350 mg/dl. Tolerated and rejected grafts were studied histologically and by immunostaining for human T-cells and insulin production. CD4 and CD8 T-cells from the spleen were studied for suppressor activity, expression of cytokines, and CD40L. Although human T-cell engraftment was similar in all groups, ILT3-Fc-treated mice tolerated the islets for the entire period of observation (91 days), whereas control mice rejected the graft within 7 weeks (P < 0.0001). ILT3-Fc treatment suppressed the expression of cytokines and CD40L and induced the differentiation of human CD8(+) T suppressor cells that inhibited Th alloreactivit...Continue Reading

References

Jul 1, 1990·Acta diabetologica latina·C RicordiR Alejandro
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·N S KenyonC Ricordi
Dec 22, 1999·Clinical and Experimental Immunology·K G TournoyG G Leroux-Roels
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E J WagarD L Greiner
Jan 13, 2001·Current Opinion in Immunology·S Gallucci, P Matzinger
Jan 26, 2002·Transplantation·Pierre Y Benhamou
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nancy E PhillipsAldo A Rossini
Aug 17, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Scott J BanuelosMichael C Appel
Jan 8, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Hirohito IchiiCamillo Ricordi
Feb 4, 2005·Diabetologia·D KleinR L Pastori
Apr 20, 2005·The Journal of Experimental Medicine·Silvia GregoriMaria-Grazia Roncarolo
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·George VladNicole Suciu-Foca
Nov 23, 2005·The Journal of Experimental Medicine·Francesca MacchiariniLeonard D Shultz
Feb 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seunghee Kim-SchulzeNicole Suciu-Foca
Apr 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C P LarsenA D Kirk
Jan 30, 2007·Nature Reviews. Immunology·Leonard D ShultzDale L Greiner
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicole Suciu-FocaRaffaello Cortesini
Sep 12, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P M CampbellA M J Shapiro
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Anthony HoganCamillo Ricordi
Dec 22, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Marie KingDale L Greiner

❮ Previous
Next ❯

Citations

Aug 13, 2010·The Review of Diabetic Studies : RDS·Vijayaganapathy VaithilingamBernard E Tuch
Feb 12, 2014·Nature Reviews. Nephrology·Desiree Tampe, Michael Zeisberg
Oct 1, 2010·Current Opinion in Organ Transplantation·Carole GuillonneauIgnacio Anegon
Jun 16, 2009·Expert Opinion on Biological Therapy·Christine KonyaCornelia M Weyand
Sep 29, 2012·Experimental and Molecular Pathology·George Vlad, Nicole Suciu-Foca
Oct 27, 2010·Human Immunology·George VladNicole Suciu-Foca
Mar 12, 2009·Human Immunology·George VladNicole Suciu-Foca
May 1, 2009·Human Immunology·Christian A Gleissner, Thomas J Dengler
Sep 27, 2008·Human Immunology·George VladNicole Suciu-Foca
Oct 3, 2012·Cytometry. Part B, Clinical Cytometry·Hanna DobrowolskaAdriana I Colovai
Apr 14, 2010·Scandinavian Journal of Immunology·S JacobsonM Flodström-Tullberg
Jun 27, 2009·Human Immunology·Eric K HoDonna Mancini
Aug 6, 2011·Cell Stem Cell·Bonnie Barrilleaux, Paul S Knoepfler
Dec 22, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zheng XuNicole Suciu-Foca
Apr 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chih-Chao ChangNicole Suciu-Foca
Jan 11, 2019·Frontiers in Immunology·Sandeep KumarXuefang Cao
Dec 8, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rebecca G BreslowHoward R Katz
Dec 15, 2016·Frontiers in Immunology·Zheng XuNicole Suciu-Foca
Mar 6, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chih-Chao ChangGeorge Vlad
Oct 12, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chih-Chao ChangNicole Suciu-Foca
Dec 29, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zheng XuNicole Suciu-Foca
May 1, 2021·Antibody Therapeutics·Mi DengCheng Cheng Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
electrophoresis
PCR
flow-
cytometry

Software Mentioned

7300 SDS
BMDP

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.