Abstract
Radioimmunoassays for IgM and IgG histoplasmal antibodies were developed and proved to be specific for their respective immunoglobulin classes, sensitive, and reproducible. Elevated IgM antibodies were detected in 59.8% of patients with histoplasmosis and 7.9% of control subjects. Elevated IgG antibodies occurred in 80.4% of patients with histoplasmosis but in only 12.9% of control subjects. Radioimmunoassay was nearly twice as sensitive as complement fixation for identifying patients with mild, presumably asymptomatic, infection. Of 13 patients with serologic follow-up at least 1 yr later, elevated IgM antibodies cleared, whereas IgG antibodies persisted in 7. In an epidemiologic investigation of a recurrent histoplasmosis outbreak, only the radioimmunoassay was able to prove the hypothesis that construction for a swimming complex was the source of exposure. These assays promise to be useful for clinical and epidemiologic investigations.
Citations
Jan 23, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sarah M RicherL Joseph Wheat
Jan 1, 1992·The American Journal of Medicine
Jan 1, 1990·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·A J HamiltonR J Hay
Feb 1, 1985·The American Journal of Medicine·L J WheatM L Zeckel
Jan 1, 1990·Journal of Clinical Laboratory Analysis·C RamanR P Tewari
Mar 10, 2017·Journal of Clinical Microbiology·Marwan M Azar, Chadi A Hage
Aug 18, 2006·Transplant Infectious Disease : an Official Journal of the Transplantation Society·L J Wheat
Nov 24, 1999·Allergy·E Millqvist
Apr 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L de Repentigny
Oct 20, 2006·Expert Opinion on Biological Therapy·L Joseph Wheat
Apr 17, 1999·Medical Mycology·A J Hamilton
Dec 23, 1999·The Pediatric Infectious Disease Journal·C M OdioA Carranza
Jun 9, 2020·Biocontrol Science·Haruna SasakiHiroshi Morita
Aug 1, 1997·American Journal of Respiratory Cell and Molecular Biology·J H PersoonsG Kraal
Jan 16, 2007·Clinical Microbiology Reviews·Carol A Kauffman
Jan 9, 1986·The New England Journal of Medicine·L J WheatR P Tewari
Jan 2, 2021·Journal of Fungi·Kathleen A Linder, Carol A Kauffman
Nov 1, 1993·Infection and Immunity·M F FojtasekC T Schnizlein-Bick
Jan 1, 1990·Journal of Clinical Microbiology·S E ZimmermanL J Wheat
Mar 1, 1989·Infection and Immunity·S M KamelR P Tewari
May 1, 1997·Journal of Clinical Microbiology·R ChandrashekarG J Weil
Mar 1, 1986·Journal of Clinical Microbiology·J WheatR P Tewari
Oct 15, 2003·Trends in Microbiology·L Joseph Wheat