Immunoglobulin M (IgM)-glycoinositolphospholipid enzyme-linked immunosorbent assay: an immunoenzymatic assay for discrimination between patients with acute toxoplasmosis and those with persistent parasite-specific IgM antibodies.

Journal of Clinical Microbiology
Mónica GiraldoRicardo T Gazzinelli

Abstract

In the present study we developed an enzyme-linked immunosorbent assay (ELISA) to measure immunoglobulin M (IgM) specific for glycoinositolphospholipids (GIPL) derived from tachyzoite membrane (IgM-GIPL ELISA). The sensitivity and specificity of the assay were compared with those of commercially available Toxoplasma-specific IgM serological tests, namely, immunofluorescence assay (IFA) with fixed tachyzoites and capture ELISA employing tachyzoite extracts. Our results show that all patients with acute toxoplasmosis, as determined by clinical data and conventional serological tests, were also positive by the IgM-GIPL ELISA. Interestingly, many patients that were classified as indeterminate, who had IgG with high avidity but positive results in the IgM-specific IFA and capture ELISA, were negative by the IgM-GIPL ELISA. Finally, we tested the sera from patients with rheumatoid arthritis and various parasitic infections and found no evidence of false positives in the IgM-GIPL ELISA.

References

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Citations

Apr 12, 2012·Clinical Microbiology Reviews·Florence Robert-Gangneux, Marie-Laure Dardé
Dec 1, 2007·Expert Opinion on Medical Diagnostics·Stuart L Hazell
Nov 12, 2014·Schizophrenia Bulletin·Joel M MonroeBrian J Miller
May 17, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A Sensini
Apr 18, 2006·Microbes and Infection·Elaine V LourençoMaria-Cristina Roque-Barreira
Dec 10, 2002·Memórias do Instituto Oswaldo Cruz·Alessandra Carla de Almeida RibeiroJosé Roberto Mineo
Jul 28, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Miriam Flores RebouçasRicardo Wagner Portela

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