Immunogold cytochemistry identifies specialized membrane domains for monocarboxylate transport in the central nervous system

Neurochemical Research
Linda BergersenOle Petter Ottersen

Abstract

An efficient exchange of lactate between different cell types (such as astrocytes and neurones) would require that lactate transporters are expressed in contiguous parts of the respective plasma membranes. To settle this issue we explored the subcellular expression pattern of monocarboxylate transporters (MCTs) by use of selective antibodies and high resolution immunogold cytochemistry. We investigated whether the membrane domains containing MCT1, MCT2 and MCT4 are spatially related to each other and to other membrane domains, i.e. those containing glutamate receptors. We used retina and cerebellum as a model for our investigations. We found that MCT1 was localized in the apical membrane of pigment epithelial cells and in the photoreceptor inner segment membrane in the retina. In the brain MCT1 was present in endothelial cells. MCT2 was localized in the postsynaptic membrane of parallel fiber-Purkinje cell synapses and MCT4 was situated in the membrane of glial cells in the cerebellum.

Citations

Aug 14, 2003·American Journal of Physiology. Endocrinology and Metabolism·Raymond MengualMichele Beaudry
Dec 20, 2003·Nature Reviews. Neuroscience·Mahmood Amiry-Moghaddam, Ole P Ottersen
Nov 6, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Luc Pellerin, Pierre J Magistretti
Dec 9, 2004·Journal of Neuroscience Research·Leif Hertz, Gerald A Dienel
Apr 23, 2005·Glia·Gerald A Dienel, Leif Hertz
Oct 2, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Camille Robinet, Luc Pellerin
Dec 22, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gerald A Dienel
Mar 24, 2017·International Journal of Molecular Sciences·Maria Cláudia de B LuzBeatriz da C A Alves
Apr 6, 2017·Frontiers in Endocrinology·Alejandra Freire-RegatilloJulie A Chowen
Oct 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Susan J Vannucci, Ian A Simpson
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Eain M Cornford, Shigeyo Hyman
Jun 15, 2005·Journal of Neurochemistry·Karin Pierre, Luc Pellerin
May 16, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Qi Wang, Marilyn E Morris
Jul 22, 2014·Journal of Neurochemistry·Philip W BeesleyConstanze Seidenbecher
Sep 23, 2020·Journal of Neuroinflammation·Daniel C ShippyTyler K Ulland
Jun 30, 2007·The Journal of Physiology·Karin PierreLuc Pellerin
Aug 19, 2007·Metabolic Brain Disease·Jérôme BadautLuca Regli
Jun 21, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ian A SimpsonSusan J Vannucci
Feb 4, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anant B PatelGraeme F Mason
Mar 28, 2012·The Journal of Physiology·Malin H StridhHolger M Becker
Dec 6, 2017·The European Journal of Neuroscience·Sonja BroselLars Kunz
Sep 10, 2004·The European Journal of Neuroscience·Bang V BuiMichael Kalloniatis
Jul 18, 2012·Nature·Youngjin LeeJeffrey D Rothstein
Jan 15, 2014·Global Advances in Health and Medicine : Improving Healthcare Outcomes Worldwide·Derrick Macfabe
Jan 27, 2019·Journal of Neuroinflammation·Marta A MałkiewiczPaweł J Winklewski
Jan 20, 2018·Physiological Reviews·Alexei Verkhratsky, Maiken Nedergaard
Feb 10, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lasse K BakHelle S Waagepetersen
Aug 11, 2020·Progress in Retinal and Eye Research·Meysam YazdankhahNadezda A Stepicheva

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