Immunogold-labeled S-phase neoblasts, total neoblast number, their distribution, and evidence for arrested neoblasts in Macrostomum lignano (Platyhelminthes, Rhabditophora)

Cell and Tissue Research
A BodeP Ladurner

Abstract

Neoblasts in Platyhelminthes are the only cells to proliferate and differentiate into all cell types. In Macrostomum lignano, the incorporation of 5'-bromo-2'-deoxyuridine (BrdU) in neoblasts confirmed the distribution of S-phase cells in two lateral bands. BrdU labeling for light and for transmission electron microscopy (TEM) identified three populations of proliferating cells: somatic neoblasts located between the epidermis and gastrodermis (mesodermal neoblasts), neoblasts located within the gastrodermis (gastrodermal neoblasts), and gonadal S-phase cells. In adults, three stages of mesodermal neoblasts (2, 2-3, and 3) defined by their ultrastructure were found. Stage 1 neoblasts where only seen in hatchlings. These stages either were phases within the S-phase of one neoblast pool or were subsequent stages of differentiating neoblasts, each with its own cell cycle. Regular TEM and immunogold labeling provided the basis for calculating the total number of neoblasts and the ratio of labeled to non-labeled neoblasts. Somatic neoblasts represented 6.5% of the total number of cells. Of these, 27% were labeled in S-phase. Of this fraction, 33% were in stage 2, 46% in stage 2-3, and 21% in stage 3. Immunogold labeling substantiated...Continue Reading

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Citations

Oct 18, 2006·Cell and Tissue Research·Katharina Theresia NimethRobert Gschwentner
Dec 21, 2007·Cell and Tissue Research·Natalia D KreshchenkoMargaretha K S Gustafsson
May 18, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sanjeev K Waghmare, Tudorita Tumbar
Dec 19, 2009·BMC Developmental Biology·Katrien De MulderPeter Ladurner
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May 12, 2009·PloS One·Bernhard EggerPeter Ladurner
Feb 4, 2010·Cell and Tissue Research·Katrien De MulderPeter Ladurner
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Jul 28, 2009·Developmental Biology·Katrien De MulderPeter Ladurner
Jun 4, 2016·BMC Developmental Biology·Birgit LengererPeter Ladurner
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Feb 4, 2010·Journal of Cell Science·Lucia BonuccelliAlessandra Salvetti

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