Immunogold localisation of endogenous immunoglobulin-G in ultrathin frozen sections of the human placenta

Cell and Tissue Research
L LeachS F Contractor

Abstract

Endogenous immunoglobulin-G was localised in ultrathin frozen sections of human term placenta by use of an indirect immuno electron-histochemical methodology. Immunoreactivity of endogenous IgG to rabbit anti-human immunoglobulin-G antibody was visualised by use of protein-A--colloidal gold complex. Gold marked the syncytiotrophoblast in both coated and uncoated regions of the apical plasmalemma, in vesicles and multivesicular bodies, and in vesicles near the basal plasmalemma. Immunoreactivity was also seen in the interstitial space between the trophoblast and the fetal endothelial layer as well as in various types of vesicles within the endothelial cells. No immunoreactivity was seen in the intercellular clefts of the endothelium. The pattern of localisation observed is consistent with receptor-mediated uptake of immunoglobulin-G into the syncytiotrophoblast of the human placenta followed by release into the interstitial space and then vesicular transport through the endothelium.

References

Jun 1, 1978·Journal of Ultrastructure Research·K T Tokuyasu
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M BundgaardC Crone
Dec 1, 1976·The Journal of Cell Biology·K T Tokuyasu, S J Singer
Apr 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K L GoldenthalM C Willingham
Nov 15, 1985·The Biochemical Journal·T WilemanP Stahl
Feb 1, 1985·Journal of Applied Physiology·E M Renkin
May 1, 1973·The Journal of Cell Biology·N SimionescuG E Palade
Sep 1, 1983·Journal of Reproductive Immunology·S F ContractorP J Stannard
Apr 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C T Lin
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B M Pearse
Oct 1, 1981·Placenta·P M Johnson, P J Brown
Oct 1, 1964·The Journal of Clinical Investigation·D GITLINC MORALES

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Citations

Aug 1, 1995·Cell and Tissue Research·N A Bright, C D Ockleford
Jan 1, 1994·Molecular Aspects of Medicine·G Desoye, E Shafrir
Jul 1, 1994·Placenta·F SajiN Matsuzaki
Mar 21, 1998·Journal of Reproductive Immunology·N E Simister, C M Story
Aug 1, 1994·American Journal of Reproductive Immunology : AJRI·A MalekH Schneider
Dec 3, 2013·Progress in Biophysics and Molecular Biology·Berthold HuppertzJayasree Sengupta
Nov 26, 2009·Hematology/oncology Clinics of North America·Evi Stavrou, Keith R McCrae
Sep 1, 1995·American Journal of Obstetrics and Gynecology·A MalekH Schneider
Jun 5, 2016·Nutrition Reviews·Chang Cao, Mark D Fleming
Feb 1, 1996·European Journal of Immunology·E K Kristoffersen, R Matre
Jul 1, 1996·European Journal of Immunology·E K Kristoffersen, R Matre
Jun 18, 2021·The Journal of Obstetrics and Gynaecology Research·Hongqiang LuoWeiying Feng

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