Immunogold localisation of the intermediate chain within the protein complex of cytoplasmic dynein

Journal of Structural Biology
W SteffenG Wiche

Abstract

It was our goal to determine the location of the intermediate chain within the complex of cytoplasmic dynein by immunoelectron microscopy. To do so we generated two monoclonal antibodies (m74-1 and m74-2) specific for the intermediate chain. Both antibodies recognised the intermediate chain by sodium dodecyl sulphate-polyacrylamide gel electrophoresis immunoblot and ELISA assays of native and denatured proteins. When sucrose density gradient-purified cytoplasmic dynein from bovine brain was incubated with the gold-conjugated monoclonal antibodies, m74-1 and m74-2, and examined by negative staining, the gold label was found opposite the globular heads at the base of the V-shaped stalk of the motor complex. The labelling of the intermediate chain is the first mapping of a component within cytoplasmic dynein. The identification of the intermediate chain at the base of the complex supports a possible docking function of the intermediate chain.

Citations

Feb 7, 1998·Cell Motility and the Cytoskeleton·M P Koonce, D A Knecht
Oct 24, 1998·Cell Motility and the Cytoskeleton·K K PfisterS M King
Dec 22, 2000·Cell Motility and the Cytoskeleton·H NilssonR E Palazzo
Dec 22, 2000·Cell Motility and the Cytoskeleton·A R Kini, C A Collins
Sep 14, 2011·The Journal of Biological Chemistry·Richard J McKenneyRichard B Vallee
Sep 29, 2012·The Journal of Biological Chemistry·Maike H HinrichsTim Scholz
Mar 22, 2002·Molecular Biology of the Cell·Paula M GrissomJ Richard McIntosh
Oct 8, 2004·Molecular Biology of the Cell·Triscia W HendricksonWinfield S Sale
Jan 28, 2006·PLoS Genetics·K Kevin PfisterElizabeth M C Fisher
Sep 6, 2002·Pigment Cell Research·Helén Nilsson SköldMargareta Wallin
Sep 16, 2015·Microscopy·Shiori TobaShinji Hirotsune
Feb 11, 2009·IEEE Transactions on Nanobioscience·Zhiliang JiangXiaoling Wei
Oct 16, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Zhi-Liang JiangAi-Hui Liang
Apr 20, 2010·Current Biology : CB·Harry W SchroederYale E Goldman
Mar 10, 2015·Nature Structural & Molecular Biology·Saikat ChowdhuryGabriel C Lander
Jul 6, 2004·Cell Motility and the Cytoskeleton·Shiori Toba, Yoko Yano Toyoshima
Oct 7, 2004·Cell Motility and the Cytoskeleton·Siming LiuDavid G Pennock
Dec 24, 2018·FEBS Letters·Vincent A BehrensWalter Steffen
May 25, 2006·Journal of Neuroscience Research·Huei-Hsuan ChengYen-Chung Chang
Jun 22, 2002·The Journal of Biological Chemistry·Stephen J SusalkaK Kevin Pfister
Jan 25, 2002·The Journal of Cell Biology·Olga KrylyshkinaJ Victor Small
May 8, 2001·The Journal of Biological Chemistry·P S VaughanK T Vaughan
Mar 20, 2008·Journal of Biomolecular Screening·Zhiliang JiangMianwu Meng
Jan 3, 2001·Methods : a Companion to Methods in Enzymology·L B Brill, K K Pfister
Mar 21, 2000·Biochimica Et Biophysica Acta·S M King
Mar 21, 2000·Biochimica Et Biophysica Acta·R B ValleeC Y Tai

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