PMID: 8609371Feb 1, 1996Paper

Immunogold probes for light and electron microscopic localization of Ole e I in several Oleaceae pollens

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
M C FernándezM I Rodríguez-García

Abstract

We investigated the immunolocalization of the olive major allergen Ole e I and Ole e I-like proteins in pollen from several Oleaceae species [olive (Olea europaea), ash (Fraxinus excelsior), privet (Ligustrum vulgaris), lilac (Syringa vulgare), and forsythia (Forsythia suspensa)]. Crossreactions among different pollens were found in enzyme immunoassays. For immunolocalization with light microscopy we used the silver enhancement technique with three monoclonal antibodies (1D8, 10H1, and 16G2) that recognize three different epitopes of the allergen Ole e I. Our findings show that the silver enhancement technique is very useful when several antibodies are to be used for rapid screening of different materials. MAb 10H1 gave the most precise results and was selected for further immunolocalization studies with transmission electron microscopy. The epitope recognized by this MAb was localized exclusively in the endoplasmic reticulum in olive pollen. In lilac, privet, and ash pollen, most of the reactivity was also seen in the endoplasmic reticulum; however, the 10H1 epitope was not detected in forsythia pollen.

Citations

Jul 28, 2001·Micron : the International Research and Review Journal for Microscopy·J D AlchéM I Rodríguez-García
Jan 11, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C De LinaresM M Trigo

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