Immunohistochemical- and PCR-based assay for the reproducible, routine detection of erythrovirus B19 in thyroid tissues

Journal of Medical Virology
Cyril PageGilles Duverlie

Abstract

It is generally accepted that thyroid follicle cells are at least semi-permissive for erythrovirus B19 (EVB19). Thus, various laboratory techniques have been successfully used to detect EVB19 in the thyroid gland, including polymerase chain reaction (PCR), immunohistochemistry, and in situ hybridization. However, the detection of EVB19 within the thyroid gland is problematic, and none of the detection protocols in the literature have been unequivocally validated. This multidisciplinary study in which 32 thyroidectomy subjects undergoing thyroidectomy in a French University hospital were prospectively recruited was performed over a period of 3 years. Prior to surgery, all the subjects were assayed for blood levels of anti-EVB19 antibodies and (using a quantitative PCR [qPCR] assay) EVB19 itself. A qPCR assay for EVB19 and an immunohistochemical assay (based on polyclonal anti-VP2 antibodies) were performed on the thyroidectomy samples. None of the subjects had an acute EVB19 infection. A viral load was detected in two serum samples and six thyroid biopsies. Three subjects had both a positive immunohistochemical assay and a positive qPCR assay for the thyroid tissue. It is noteworthy that the thyroid immunohistochemical and qPCR ...Continue Reading

References

Mar 18, 2003·Joint, Bone, Spine : Revue Du Rhumatisme·Olivier Meyer
May 8, 2004·Journal of Clinical Microbiology·Kati HokynarMaria Söderlund-Venermo
Jun 22, 2007·The Journal of Infection·Kouki MoriKatsumi Yoshida
Jan 24, 2008·British Journal of Cancer·J H WangG S Huang
Jan 14, 2009·Virology Journal·Rachel Desailloud, Didier Hober
Feb 16, 2010·The Journal of Infection·Juanhong WangGaosheng Huang
Dec 31, 2010·Thyroid : Official Journal of the American Thyroid Association·Laura A AdamsonJacqueline A Hobbs
Nov 23, 2013·Journal of Medical Virology·Laura A AdamsonJacqueline A Hobbs

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Citations

Jan 19, 2016·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·A Servant-Delmas, F Morinet
May 26, 2016·Reviews in Endocrine & Metabolic Disorders·Poupak FallahiAlessandro Antonelli
Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ashkan EtemadiMohsen Moghoofei
Apr 5, 2017·Frontiers in Endocrinology·Silvia Martina FerrariSalvatore Benvenga
Feb 29, 2020·Best Practice & Research. Clinical Endocrinology & Metabolism·Alessandro AntonelliPoupak Fallahi

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