Immunohistochemical analysis of human growth hormone-releasing hormone gene expression in transgenic mice

Endocrinology
A K BrarL A Frohman

Abstract

The tissue-specific expression of a fusion gene encoding the mouse metallothionein-1 promoter and the coding region of the human GH-releasing hormone (hGRH) gene was studied in transgenic mice by immunohistochemistry using an anti-hGRH serum that does not recognize endogenous mouse GRH. hGRH immunoreactivity (GRH-IR) was detected in specific cells of the pituitary, pancreas, kidney, duodenum, lung, testis, ovary, adrenal, heart, and brain. In the pituitary, using double immunofluorescent staining, GRH-IR was found in some, but not all, somatotrophs, gonadotrophs, thyrotrophs, and mammotrophs. GRH-IR was found in both pancreatic exocrine cells and endocrine islets. Within the islet, GRH-IR was colocalized in A and D cells with glucagon and somatostatin, respectively. Immunopositive cells in other tissues were localized in kidney proximal convoluted tubules, duodenal submucosal glands of Brunner, the smooth muscles of pulmonary arterioles, testicular Leydig cells, oocytes, adrenal medullary chromaffin cells, and cardiac atria. In the brain, GRH-IR was seen in the external layer of the median eminence and in perikarya and fibers of the hypothalamic arcuate nucleus, the parvocellular region of the paraventricular nucleus, the supra...Continue Reading

Citations

Nov 15, 1997·Microscopy Research and Technique·L Stefaneanu, K Kovacs
Aug 7, 2002·Trends in Endocrinology and Metabolism : TEM·Lawrence A Frohman, Rhonda D Kineman
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S González-Crespo, A Boronat
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·M O ThornerC Y Bowers
Jan 1, 1990·Acta Paediatrica Scandinavica. Supplement·L A FrohmanM A Frohman
Feb 1, 1995·Kidney International·P BriandA Vandewalle
Jun 1, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yu-Hong HuangRyohachi Arai
Oct 1, 2004·The Journal of Biological Chemistry·Siwanon JirawatnotaiHiroaki Kiyokawa
Sep 1, 1993·Endocrine Pathology·Ricardo V LloydSally A Camper
Feb 26, 2015·Endocrine-related Cancer·Albert BeckersConstantine A Stratakis
Aug 1, 1996·Metabolism: Clinical and Experimental·L A Frohman

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