PMID: 2503520Jan 1, 1989Paper

Immunohistochemical and biochemical characterization of antisera raised to human tumor nucleoli

Journal of Cancer Research and Clinical Oncology
R S GreenfieldJ A Grattan

Abstract

Antinucleolar antisera were raised in rabbits, goats and sheep to nucleoli isolated from three human tumor cell lines. The antisera were shown to cross-react by immunofluorescence with human tumor cell lines originating from different organs and with frozen sections from a wide variety of human malignant and non-malignant tissues. Tumor versus normal tissue discrimination by several antisera was significantly improved by treatment of frozen tissues with a buffered glutaraldehyde/Triton X-100 solution prior to immunofluorescent staining. The molecular specificity of these antisera was determined by immunostaining electrotransfer nitrocellulose strips following SDS-PAGE of nucleolar preparations and nuclear extracts. Although different immunostaining patterns were obtained for individual antinucleolar antisera, nucleolar proteins of molecular weight 120, 100, 94, 68, 54, 38, 33, and 32 kDa were the most often recognized by antisera raised in the three different species. G187 antiserum strongly reacted with 100, 94, and 38 kDa proteins from freshly obtained leukemic specimens. The Immunoreactivity of the 100, 94, and 38 kDa proteins was unaffected by glutaraldehyde/Triton X-100 treatment when immunostained with antisera that demon...Continue Reading

References

May 9, 1977·Biochemical and Biophysical Research Communications·M D MamrackH Busch
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F M DavisH Busch
Jul 16, 1979·Life Sciences·K SmetanaH Busch
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1984·The Journal of Cell Biology·J KistlerU K Laemmli
Jan 1, 1982·Journal of Cancer Research and Clinical Oncology·P K ChanH Busch

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Citations

Jan 1, 1994·Leukemia & Lymphoma·F L ErdkampG H Blijham
Sep 2, 2004·Journal of Addictive Diseases·Alfred S FriedmanJean McCallum

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