PMID: 7539568Jan 1, 1995Paper

Immunohistochemical and glycohistochemical localization of the beta-galactoside-binding S-type lectin in human placenta

Acta histochemica
Hermann WalzelJosef Brock

Abstract

The localization of the beta-galactoside binding lectin was studied immunohistochemically on acetone-fixed cryostat sections of full-term placental tissue using a biotinylated monoclonal antibody and glycohistochemically applying biotinylated asialofetuin and lactosylated bovine serum albumin. On blots of placental tissue lysates the lectin is recognized by the biotinylated lactosylated bovine serum albumin. The glycoconjugate recognition of the lectin on blots was inhibited in the presence of 0.1 M lactose showing the specificity of the interactions. The anti-lectin monoclonal antibody stained syncytiotrophoblast and trophoblastic cells. Both reagents applied for glycohistochemistry stained syncytiotrophoblast and trophoblastic cells of placental villi and the trophoblastic layer of extraplacental membranes. A strong uniform cytoplasmic staining was characteristic for syncytiotrophoblast and to a lower extent for cytotrophoblastic cells. The localization of the lectin is discussed with respect to a possible immunosuppressive function.

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Citations

Oct 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nandor Gabor ThanDerek E Wildman
Sep 25, 2012·Molecular Immunology·David C Kilpatrick
May 29, 2015·Journal of Pathology and Translational Medicine·Nandor Gabor ThanChong Jai Kim
Jun 3, 2011·Journal of Reproductive Immunology·Isabelle FischerAlexander G Betz
Jun 24, 2008·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Nandor Gabor ThanRoberto Romero
Jul 9, 2008·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Francesca GotschSurendra Sharma

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