Immunohistochemical and ultrastructural study of adult porcine endocrine pancreas during the different steps of islet isolation

Histochemistry and Cell Biology
M C VantyghemJ C Beauvillain

Abstract

Treatment of diabetes mellitus by transplantation of isolated pancreatic islets could constitute an alternative to human pancreas allograft. Before transplantation, porcine islets are submitted to a procedure of isolation and purification. The quality of islets through these different steps may be assessed by morphological and functional studies. The aim of this work was the histological characterization of the four main cell types of porcine adult endocrine islets during the different steps of the isolation procedure using immunohistochemistry (IHC) applied in light (LM) and electron microscopy (EM). In fresh pancreas, islets were various sizes and shapes in LM. The number was not found different between the different portions of the pancreas. In IHC, insulin (Ins)-secreting cells accounted for the majority of the islet cells, while glucagon(Glu)-somatostatin (Som)- and polypeptide(PP)-immunoreactive cells, in decreasing number, were found in the mantle around the core of Ins-cells. In EM, B-cells contained poly-hedric granules with a dense central core and clear halo. Glu granules were spherical and very dense. D-cells and PP-cells were characterized by numerous granules, rather spherical and of inequal density for Som and mo...Continue Reading

References

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Citations

Apr 16, 2003·Cell Transplantation·T B NielsenH Beck-Nielsen
Mar 17, 2001·Diabetic Medicine : a Journal of the British Diabetic Association·S A WhiteM L Nicholson
Apr 9, 1998·Transplantation Proceedings·M C VantyghemM Mazzuca
Sep 12, 2021·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth KemterMichele Solimena

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