Immunohistochemical changes and atrophy after chronic ethanol intoxication in rat salivary glands

Histology and Histopathology
Luanna Melo Pereira FernandesRafael Rodrigues Lima

Abstract

Alcoholism in humans is a chronic and progressive disease, characterized by loss of ethanol consumption control. Previous studies have reported that prolonged exposure to ethanol was responsible for alterations in glandular tissues of human and rodents. However, the interrelationship between ethanol and the glandular system is still the subject of numerous investigations, including the possible resistance of the submandibular gland (SG). In the present study, we investigated whether chronic ethanol exposure during adolescence may affect the parotid gland (PG) and SG in female rats. Female rats (n=16) were treated with distilled water or ethanol (dose of 6.5 g/kg/day, 22.5% w/v) through gavage for 55 days. Glands were collected, weighed and submitted to histological processing. Morphometric analysis was assessed by parenchymal and stromal area measurements. Smooth muscle actin (α-SMA), cytokeratin-19 (CK19) and apoptotic caspase-3 (CAS) were measured using ImageJ® software. Chronic ethanol administration did not alter the body weight of rats after treatment, although it increased glandular weight (p<0.001), reduced the parenchyma area (p<0.001) and decreased CK19 and α-SMA immunostainning in the PG. Besides, ethanol induced CK19...Continue Reading

Citations

Sep 1, 2016·Oxidative Medicine and Cellular Longevity·Nathalia Carolina Fernandes FagundesRafael Rodrigues Lima
Oct 18, 2018·Oxidative Medicine and Cellular Longevity·Luanna Melo Pereira FernandesCristiane Socorro Ferraz Maia
Jan 17, 2017·Oxidative Medicine and Cellular Longevity·Rafael R LimaWalace Gomes-Leal
Oct 15, 2021·Frontiers in Pharmacology·Leidiane Alencar de Oliveira LimaRafael Rodrigues Lima

❮ Previous
Next ❯

Related Concepts

Related Feeds

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.

Autoimmune Lymphoproliferative Syndrome

Autoimmune lymphoproliferative syndrome (ALPS) is a rare genetic disorder of abnormal lymphocyte survival caused by defective Fas mediated apoptosis. Discover the latest research on ALPS here.