PMID: 8938947Jan 1, 1996Paper

Immunohistochemical characterization of 22 monoclonal antibodies against the CA125 antigen: 2nd report from the ISOBM TD-1 Workshop

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
M NapJ Hilgers

Abstract

We evaluated the immunohistological (IH) characteristics of 22 different antibodies that were submitted for study in the frame of the TD-1 ISOBM Workshop on monoclonal antibodies against CA125. Information on relative affinities and epitope similarities was obtained from a parallel immunochemical study. Antibodies were tested at concentrations of 10 and 1 micrograms/ml on frozen and paraffin sections. Paraffin sections were stained according to the streptavidin-biotin complex protocol, and frozen sections according to a two-step immunoperoxidase technique. Aminoethylcarbazole served as the chromogen. The tissues were from normal proliferative endometrium (formalin-fixed paraffin-embedded) material and clear-cell adenocarcinoma of the ovary (formalin-fixed paraffin-embedded and frozen material). Sections were scored for staining in epithelial cells, basal, apical and diffuse cytoplasmic and in stromal components. Intensity was graded as 1, 2 or 3 for epithelial cells and as -1, -2 or -3 for stroma. The cumulative scores for each antibody expressed the discriminative properties of specific epithelial staining against background. M11 and M11-like antibodies, as well as OC125 and OC125-like antibodies, in general showed good staini...Continue Reading

Citations

Sep 13, 2003·Journal of Immunological Methods·Birgit C Schultes, Theresa L Whiteside
Mar 27, 2007·Bioinformatics·George VasmatzisFarhad Kosari
Apr 13, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nathalie SchollerNicole Urban
Dec 1, 2007·Biomarkers in Medicine·Nathalie Scholler, Nicole Urban
Sep 10, 2003·Molecular Biology of the Cell·Marci E SchanerBranimir I Sikic
Aug 3, 2005·Gynecologic Oncology·Liping HanZhenhe Suo
Aug 3, 2005·Gynecologic Oncology·Daniel G RosenRobert C Bast
Feb 7, 2004·The Journal of Biological Chemistry·Takeshi MaedaShigeyuki Yokoyama
Jul 13, 2018·Expert Opinion on Therapeutic Targets·Abhijit AithalSurinder K Batra
Apr 14, 2006·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Judith SingletonEarl F Albone
Feb 9, 2010·Immunological Investigations·Sorour ShojaeianMahmood Jeddi-Tehrani

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