Abstract
The epithelial mucin MUC1 is heavily but differently glycosylated depending on the origin and developmental status of the tissue, which greatly influences the reactivity of monoclonal antibodies (MAbs). A partial characterization of their epitopes is possible by mild, carbohydrate-specific periodate oxidation of tissue sections prior to immunostaining. Using this strategy, we have evaluated 56 MAbs submitted to the ISOBM TD-4 (MUC1) Workshop. Paraffin sections from normal human small intestine, colon and breast were immunostained at different defined antibody concentrations either directly or after oxidation with 20 mM periodate at pH 5 for 30 min (PO). In addition, monolayers of T-47D breast cancer cells without PO treatment were examined in immunofluorescence. The array of observed reactivities allowed us to classify the MAbs as follows. Fourteen antibodies were found to detect MUC1 largely independent of the degree of glycosylation, and are therefore classified as pan-MUC1 MAbs (Group A). Twenty-four MAbs were nonreactive with one or more types of the examined epithelia, but became reactive after PO of the tissue sections. We have called these differentiation-dependent MUC1 MAbs (Group B). They might be especially valuable i...Continue Reading
Citations
Nov 28, 2000·International Journal of Cancer. Journal International Du Cancer·K P KearseA E Kellogg-Wennerberg
Feb 18, 2006·Cancer Immunology, Immunotherapy : CII·Antje DanielczykSteffen Goletz
Mar 3, 2009·Hybridoma·Seyed Hamid Aghaee BakhtiariMohammad Javad Rasaee
Nov 27, 2004·Journal of Virology·Jaclyn R StonebrakerRaymond J Pickles
Feb 21, 2006·Biochemistry. Biokhimii︠a︡·S J Golubović, Z T Bojić-Trbojević
Dec 1, 2001·Journal of Clinical Pathology·R L ten BergeC J Meijer
Oct 24, 2001·In Vitro Cellular & Developmental Biology. Animal·P Meleady, M Clynes
Aug 21, 2012·PloS One·Priyadarsini KumarGordon C Douglas
Apr 20, 2010·Pathology, Research and Practice·Xiao-Na FanYi Cao
Jun 10, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Andreas KuemmelRainer Wiewrodt
Aug 15, 2008·Tissue Antigens·I V BalyasnikovaS M Danilov
Oct 14, 2003·Pathology·Chooi-Fun LeongHussin Noor Hamidah
May 10, 2002·The American Journal of Pathology·Paula HenderikxHennie R Hoogenboom
May 6, 2008·British Journal of Cancer·J CreaneyM J Millward
Dec 6, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Cord LangnerRichard Zigeuner
Jan 15, 2010·Biomarker Insights·Hongjun Jin, Richard C Zangar
Jun 22, 1999·The Journal of Biological Chemistry·S MüllerF G Hanisch