Immunohistochemical characterization of cancer-associated fibroblasts at the primary sites and in the metastatic lymph nodes of human intrahepatic cholangiocarcinoma

Human Pathology
Rei Atono ItouJiro Fujimoto

Abstract

Cancer-associated fibroblasts (CAFs) are an important constituent of the cancer stroma. In intrahepatic cholangiocarcinoma (ICC), the features of CAFs at the primary site and in the metastatic lymph nodes (Met-LNs) and their origin have been unclear. In the present study, we characterized CAFs at the primary site (n = 42) and in the Met-LNs (n = 10) of human ICC by immunohistochemistry using potential molecular markers of CAFs, portal fibroblasts (PFs), hepatic stellate cells (HSCs), and bone marrow-derived fibrocytes (BMDFs). At the primary site, the stroma was strongly positive for α-smooth muscle actin (α-SMA; marker for CAFs), platelet-derived growth factor receptor-β (PDGFR-β) (common marker for HSCs and PFs), fibulin-2, and thymus cell antigen-1 (Thy-1; PF marker), whereas immunoreactivity for fascin (HSC marker) was scarce. Most of the α-SMA-positive cells were found to express PDGFR-β, Thy-1, and fibulin-2 by double immunostaining. A small population of BMDF marker-positive (α-SMA+CD45+CD34+) cells was found by triple immunostaining. In the micro-Met-LNs, α-SMA-positive cells were absent in cancer aggregates of the LN sinus, whereas they were present in the invasion area of cancer cells from the LN sinus to the LN paren...Continue Reading

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Citations

Nov 25, 2019·Thoracic Cancer·Arik Bernard SchulzeGeorg Evers
Jan 15, 2020·Current Opinion in Gastroenterology·Javier VaqueroLaura Fouassier
Aug 28, 2019·International Journal of Molecular Sciences·Hsing-Ju Wu, Pei-Yi Chu
Jul 2, 2020·Nature Reviews. Gastroenterology & Hepatology·Jesus M BanalesGregory J Gores
Dec 17, 2020·Expert Opinion on Investigational Drugs·Juan Wang, Sumera Ilyas

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