PMID: 7008496Sep 1, 1980Paper

Immunohistochemical demonstration of alpha-1-antitrypsin in liver tissue. A methodological investigation

Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology
P P Clausen

Abstract

The influence of the tissue preparation on the immunohistochemical demonstration of alpha-1-antitrypsin (AAT) in liver tissue was evaluated using autopsy and biopsy material with and without AAT globules. On comparing frozen sections of unfixed material with paraffin sections of formalin fixed material a slightly better preservation of the immunoreactivity of AAT was observed in frozen sections. On comparing different fixatives, fixation in 10% neutral buffered formalin. Lillie's AAF or 96% ethanol/1% acetic acid caused a clearly better preservation of demonstrable AAT than fixation in Clarke's or Bouin's fixatives. The fixation time had only minor influence when using fixation times within a week. Extremely long fixation for several months caused, however, a clear reduction in demonstrable AAT. Pretreatment with proteolytic enzymes of deparaffinized sections of formalin fixed tissue caused an increase in demonstrable AAT, especially in tissues fixed for extremely long periods of time. On comparing three different immunohistochemical techniques: indirect immunoperoxidase, peroxidase/anti-peroxidase (PAP), and indirect immunofluorescnece, no convicting difference in sensitivity was observed between the three techniques. It is co...Continue Reading

References

Feb 1, 1977·Journal of Clinical Pathology·W K Blenkinsopp, G P Haffenden
Mar 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J McElrathR C Allen
Sep 1, 1975·Journal of Clinical Pathology·M B Ray, V J Desmet
Sep 1, 1974·American Journal of Clinical Pathology·P E PalmerH J Wolfe
Feb 1, 1973·Annals of Internal Medicine·J L CampraT B Reynolds
Jun 21, 1971·Annals of the New York Academy of Sciences·J Rygaard, W Olsen
Feb 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C GRAHAMM J KARNOVSKY

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