PMID: 7543118May 1, 1995Paper

Immunohistochemical demonstration of different latent membrane protein-1 epitopes of Epstein-Barr virus in lymphoproliferative diseases

Journal of Clinical Pathology
N M JiwaC J Meijer

Abstract

To compare the immunoreactivity of monoclonal antibodies S12 and CS1-4, which recognise different epitopes of the Epstein-Barr virus (EBV) latent membrane protein-1 (LMP-1), in EBV associated benign and malignant lymphoproliferative disorders and control tissues processed using different methods. Both monoclonal antibodies gave comparable results on frozen tissue sections and formalin fixed, paraffin wax embedded samples from cases with Hodgkin's disease and infectious mononucleosis. In all cases S12 stained more cells than CS1-4. For EBV associated B and T non-Hodgkin's lymphomas, frozen tissue sections yielded better LMP-1 staining results than formalin fixed material. Again, in all these cases S12 stained more cells and gave stronger results than CS1-4. For EBV negative tissues, both monoclonal antibodies showed cross-reactivity with melanocytic-like cells in the basal cell layer of the skin, synaptophysin-like staining in layers three and four of the cortex of the brain, and myelin-like staining in peripheral nerves and peripheral ganglion cells. Staining with S12 was always much stronger. Moreover, in contrast to CS1-4, S12 stained pancreatic islands in formalin fixed material but not in frozen tissue sections and sporadic...Continue Reading

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Citations

Apr 13, 2000·International Journal of Cancer. Journal International Du Cancer·I CruzC J Meijer
Apr 12, 2005·International Journal of Legal Medicine·Takaki IshikawaHitoshi Maeda
Jan 13, 2004·Journal of Virological Methods·John NichollsFriedrich A Grässer
Dec 17, 2002·Critical Reviews in Oncology/hematology·Jaap M MiddeldorpChris J L M Meijer
Oct 16, 2009·Journal of Pediatric Hematology/oncology·Sameer BakhshiPrasenjit Das
Apr 29, 2004·Journal of Clinical Pathology·A Zur HausenA J C van den Brule
Mar 13, 2012·Virology·Shih-Hung HuangNicola J Mason
Mar 27, 2002·The Annals of Otology, Rhinology, and Laryngology·Guillermo PlazaCarmen Bellas
Jun 18, 2003·The Journal of General Virology·Axel zur HausenAdriaan J C van den Brule
Feb 27, 2003·Pediatric Transplantation·Thomas G Gross, Brett J Loechelt
Oct 29, 1998·Biochemical and Biophysical Research Communications·K KasonoD T Curiel

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