Immunohistochemical demonstration of mitochondria in routinely processed tissue using a monoclonal antibody

The Journal of Pathology
W PaulusK H Wiedmann

Abstract

We report results obtained using the monoclonal antibody M-II 68, which recognizes inner mitochondrial membrane in routinely processed (formalin-fixed and paraffin-embedded) tissue by light microscopic immunohistochemistry. In ten normal brains, the range of immunoreactivity in various cell types and locations was defined. The most intense staining was observed in Purkinje cells, in neurons of cranial nerve nuclei, pons and substantia nigra, as well as in choroid plexus epithelial cells. By comparison with this control group, one case of primary mitochondrial encephalomyopathy exhibited increased staining of endothelial and vascular smooth muscle cells, choroid plexus epithelial cells, and neurons of various locations. Scattered ragged-red fibres were heavily labelled in one case of mitochondrial myopathy, while ten muscles without mitochondriopathy were left unstained. Our method is able to detect accumulations of mitochondria and increases in mitochondrial cristae density. It could prove useful for differential diagnosis of routine biopsy material and for clarification of cell types involved in mitochondrial cytopathies.

References

Jul 1, 1976·Archives of Neurology·D L SchotlandC P Lee
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1987·Cell Structure and Function·T HorikoshiK Yanagisawa
May 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C D Bedetti
Nov 1, 1988·Pathology, Research and Practice·J PeifferC Zimmermann
Dec 1, 1987·The American Journal of Physiology·F D DeMartinisK T Lampe
Jan 1, 1987·Acta Neuropathologica·E Ohama, F Ikuta
Jun 1, 1985·Annals of Neurology·S DiMauroD C DeVivo
May 1, 1973·Journal of the Neurological Sciences·L SchneckB W Volk
Mar 1, 1972·Archives of Neurology·J H FrenchC L Moore
Feb 1, 1968·Archives of Biochemistry and Biophysics·D J Arora, G de Lamirande
Feb 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L CordellD Y Mason
Jul 1, 1983·Journal of the Neurological Sciences·M A JohnsonH S Sherratt
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V M Darley-UsmarR A Capaldi
Jan 1, 1983·Journal of Inherited Metabolic Disease·S MiyabayashiY Kobayashi
Sep 1, 1982·Journal of the Neurological Sciences·J BogousslavskyF Regli

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Citations

Apr 5, 2005·Journal of Neuropathology and Experimental Neurology·Hannes Vogel, Josef Zamecnik

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