Immunohistochemical detection of 13(R)-hydroxyoctadecadienoic acid in atherosclerotic plaques of human carotid arteries using a novel specific antibody.

Acta Histochemica Et Cytochemica
Noriyuki ShibataMakio Kobayashi

Abstract

13-Hydroxyoctadecadienoic acid (13-HODE) is a major component of oxidized low density lipoprotein (OxLDL), which has been shown to have a crucial role in atherogenesis. Of the 13-HODE stereoisomers, 13(S)-HODE and 13(R)-HODE, little is known about the latter in contrast to the former. To detect 13(R)-HODE in atherosclerotic lesions, we prepared a mouse monoclonal antibody against 13(R)-HODE. Competitive enzyme-linked immunosorbent assay clarified the selective reaction of a clone mAb 13H1 with both free and bovine serum albumin-conjugated forms of 13(R)-HODE but not other oxidized lipids including 13(S)-HODE. Immunohistochemical analysis revealed the colocalization of 13(R)-HODE immunoreactivity with the OxLDL marker oxidized phosphatidylcholine immunoreactivity in vascular endothelial cells, macrophages and migrating vascular smooth muscle cells in atherosclerotic plaques of human carotid arteries. The present results provide in vivo evidence for the formation of 13(R)-HODE in atherosclerotic lesions of carotid arteries.

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Citations

Sep 11, 2012·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Christopher E RamsdenJ Douglas Mann
Jun 19, 2012·Prostaglandins & Other Lipid Mediators·Joanna Raszeja-WyszomirskaEwa Stachowska
Dec 15, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xiaolin LiuChangqing Zhang
Jul 31, 2019·Free Radical Research·Makoto KuranoYutaka Yatomi
Jul 19, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Marisol CabralJuan José Moreno

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
SMA

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