PMID: 8610103Apr 2, 1996Paper

Immunohistochemical detection of 4-hydroxynonenal protein adducts in Parkinson disease

Proceedings of the National Academy of Sciences of the United States of America
A YoritakaY Mizuno

Abstract

There is growing evidence that oxidative stress and mitochondrial respiratory failure with attendant decrease in energy output are implicated in nigral neuronal death in Parkinson disease (PD). It is not known, however, which cellular elements (neurons or glial cells) are major targets of oxygen-mediated damage. 4-Hydroxy-2-nonenal (HNE) was shown earlier to react with proteins to form stable adducts that can be used as markers of oxidative stress-induced cellular damage. We report here results of immunochemical studies using polyclonal antibodies directed against HNE-protein conjugates to label the site of oxidative damage in control subjects (ages 18-99 years) and seven patients that died of PD (ages 57-78 years). All the nigral melanized neurons in one of the midbrain sections were counted and classified into three groups according to the intensity of immunostaining for HNE-modified proteins--i.e., no staining, weak staining, and intensely positive staining. On average, 58% of nigral neurons were positively stained for HNE-modified proteins in PD; in contrast only 9% of nigral neurons were positive in the control subjects; the difference was statistically significant (Mann-Whitney U test; P < 0.01). In contrast to the substa...Continue Reading

References

Sep 1, 1992·Journal of Neurochemistry·K JellingerM B Youdim
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Uchida, E R Stadtman
Jan 1, 1991·European Neurology·M B YoudimP Riederer
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C D SmithW R Markesbery
Nov 1, 1991·Journal of Neurochemistry·D Ben-ShacharM B Youdim
Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Sep 29, 1989·Biochemical and Biophysical Research Communications·Y MizunoY Kagawa
Jun 3, 1989·Lancet·A H SchapiraC D Marsden
Feb 1, 1989·Journal of Neurochemistry·D T DexterC D Marsden
Feb 1, 1989·Journal of Neurochemistry·P RiedererM B Youdim
Oct 1, 1988·Annals of Neurology·P L McGeerE G McGeer
Dec 13, 1982·Neuroscience Letters·T L PerryS Hansen
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·S ToyokuniE R Stadtman
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R S SohalW C Orr
Oct 1, 1993·Journal of Neurochemistry·C MytilineouG Cohen

❮ Previous
Next ❯

Citations

Sep 24, 1999·Journal of Neuroscience Research·A J Bruce-Keller
Sep 29, 2000·Movement Disorders : Official Journal of the Movement Disorder Society·S BuervenichL Olson
Sep 29, 2000·Movement Disorders : Official Journal of the Movement Disorder Society·S AimeL Lopiano
Apr 1, 2003·Annals of Neurology·Peter Jenner
Apr 1, 2003·Annals of Neurology·Kevin St P McNaught, C Warren Olanow
Apr 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ranjita BetarbetJ Timothy Greenamyre
Feb 14, 2002·Archives of Biochemistry and Biophysics·Jae-Ryong KimSue Goo Rhee
May 1, 2004·Environmental Health and Preventive Medicine·Taro YamashitaMakoto Uchino
Jun 22, 2006·Neurotoxicity Research·A L Friedlich, M F Beal
Feb 12, 2010·Cellular and Molecular Life Sciences : CMLS·Qiongman Kong, Chien-Liang Glenn Lin
Jun 5, 2008·Journal of Neural Transmission·Ilse S PienaarJürgen Götz
Jun 25, 2013·Journal of Neural Transmission·Omar M E Abdel-SalamHafiza A Sharaf
Mar 12, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Sankar Surendran, Srinivasagam Rajasankar
Apr 7, 2007·Bulletin of Experimental Biology and Medicine·M Yu BobrovV V Bezuglov
Jun 22, 2012·Journal of Inherited Metabolic Disease·Zeljka KoradeNed A Porter
Apr 2, 2008·Cellular and Molecular Neurobiology·Renata P GuedesWania A Partata
Jan 1, 2000·Journal of the American Aging Association·T D Oberley, T A Zainal
Aug 18, 2012·Molecular Neurobiology·Elzbieta JandaVincenzo Mollace
Mar 24, 2004·Chemistry and Physics of Lipids·Kathleen S MontineThomas J Montine
Jun 11, 2005·Clinical Biochemistry·Dongchon Kang, Naotaka Hamasaki
May 5, 2005·Experimental Neurology·Andreas Hald, Julie Lotharius
Oct 31, 2006·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Jose W RuizThomas R Van De Water
Sep 28, 1998·Biochimica Et Biophysica Acta·K Kanazawa, H Ashida
Apr 23, 2003·Biochemical and Biophysical Research Communications·Kaori NishikawaKeiji Wada
May 30, 2002·Biochemical Pharmacology·Cesira FoppoliMaria Anna Rosei
Jun 1, 2000·Journal of the Neurological Sciences·T YamashitaM Uchino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.