PMID: 9547863Apr 21, 1998Paper

Immunohistochemical detection of 8-hydroxy-2'-deoxyguanosine in paraffin-embedded sections of rat liver after carbon tetrachloride treatment

Toxicologic Pathology
S TakahashiT Shirai

Abstract

To test the applicability of an anti-8-hydroxy-2'-deoxyguanosine (8-OH-dG) antibody for immunohistochemistry using paraffin-embedded sections, carbon tetrachloride (CCl4)-induced rat liver injury was evaluated. Male rats were given a single dose of CCl4 and killed at 6 hr, 12 hr, 1, 2, 3, and 7 days thereafter. Severe centrilobular necrosis was evident at 1 day. At 2 days, moderate mononuclear cell infiltration was present in centrilobular necrotic regions. Infiltrating mononuclear cells, surrounding sinusoidal endothelial cells and hepatocytes were stained with anti-8-OH-dG antibody at 2 and 3 days. Formation of 8-OH-dG in DNA and 8-oxo-dGTPase mRNA expression were also increased at these time points, the amounts of malondialdehyde and 4-hydroxy-2-nonenal showed 2 peaks at 6 hr and 3 days. The findings suggest that the main contributory factor in the massive hepatic necrosis was increased lipid peroxidation, rather than excessive formation of 8-OH-dG, and that the observed increase in the latter was largely due to infiltrating mononuclear cells. The agreement between biochemical data and the results for immunohistochemical analysis confirms that the anti-8-OH-dG antibody is applicable for detection of cells targeted by free ra...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Y MoM Sekiguchi
Jan 1, 1990·Methods in Enzymology·H Esterbauer, K H Cheeseman
Nov 1, 1990·Seminars in Liver Disease·A T Williams, R F Burk
Sep 25, 1987·Nucleic Acids Research·F Mauxion, M Kress
Nov 1, 1987·Chemistry and Physics of Lipids·G PoliM U Dianzani
Aug 15, 1984·The Biochemical Journal·T F Slater
Jul 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B HalliwellO I Aruoma
Dec 1, 1994·Journal of Leukocyte Biology·J A Smith

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Citations

Dec 19, 2002·Free Radical Biology & Medicine·Marcus S CookeMark D Evans
Oct 19, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·B C GottschlingA Nyska
Jan 5, 2012·World Journal of Biological Chemistry·Lisa Nicole GerjevicDuygu Dee Harrison-Findik
Sep 1, 2007·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Mary K ManibusanDavid A Eastmond
Mar 12, 2019·Journal of Pathology and Translational Medicine·Ae Ri AnMyoung Ja Chung
Apr 15, 2000·Free Radical Research·M S CookeJ Lunec
Mar 4, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·D NakaeY Konishi
Aug 7, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ricky A Sharma, Peter B Farmer

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