Immunohistochemical detection of estrogen receptor beta in alveolar bone cells of estradiol-treated female rats: possible direct action of estrogen on osteoclast life span.

Journal of Anatomy
Mady Crusodé de SouzaPaulo Sérgio Cerri

Abstract

The role of estrogen in bone resorption has been specifically related to the effect of estrogen on the signalling pathway that inhibits the formation of osteoclasts. However, osteoclast apoptosis and a significant reduction in the number of these cells have been observed in the alveolar bone of female rats treated with estradiol. In the present study, the expression of estrogen receptor beta (ERbeta) in the cells of alveolar bone was evaluated in estradiol-treated and -untreated female rats. In order to test the possible direct action of estrogen on osteoclasts, the relationship between apoptosis and ERbeta expression in these cells was also analysed. The animals received estradiol for 14 days and the alveolar bone fragments were embedded in paraffin for the quantification of tartrate-resistant acid phosphatase-positive osteoclasts. The expression of ERbeta and apoptosis in the osteoclasts were evaluated by ERbeta immunohistochemistry and Terminal deoxynucleotidyl transferase-mediated dUTP Nick-End Labelling (TUNEL) methods, respectively. To confirm osteoclast death by apoptosis, these cells were analysed under transmission electron microscopy. Some osteoclasts from estradiol-treated animals were found to be undergoing apoptosi...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
May 1, 1992·Endocrinology·J W ChowT J Chambers
Oct 12, 1973·Science·E V Jensen, E R DeSombre
Oct 1, 1996·Nature Medicine·D E HughesB F Boyce
Aug 18, 1997·The Journal of Experimental Medicine·T KamedaM Kumegawa
Sep 1, 1997·The Journal of Clinical Endocrinology and Metabolism·A TomkinsonB S Noble
Jun 1, 1997·Molecular Pathology : MP·D E Hughes, B F Boyce
Oct 24, 1997·Nature·A M BrzozowskiM Carlquist
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·O VidalC Ohlsson
Jan 11, 2000·Journal of Cellular Biochemistry·D J RickardT C Spelsberg
Jan 25, 2000·The Anatomical Record·P S CerriE Katchburian
Nov 22, 2000·The Journal of Clinical Investigation·B L Riggs
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I P BraidmanJ A Hoyland
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·B McEwenT A Milner
Aug 4, 2001·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·L C Hofbauer, A E Heufelder
Oct 3, 2001·Physiological Reviews·S NilssonJ A Gustafsson
May 23, 2002·Recent Progress in Hormone Research·S C ManolagasR L Jilka
Jan 23, 2004·American Journal of Physiology. Endocrinology and Metabolism·Jin Q ChenJames D Yager
Mar 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shao-Hua YangJames W Simpkins
Nov 17, 2004·The Journal of Biological Chemistry·Durga Prasad Mishra, Chandrima Shaha
Feb 8, 2005·Biochemical and Biophysical Research Communications·Lianping Xing, Brendan F Boyce
Feb 12, 2005·Molecular Endocrinology·Ellis R Levin
May 7, 2005·Advanced Drug Delivery Reviews·Kalervo Väänänen
Jul 28, 2005·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Paulo Sérgio Cerri
Aug 18, 2005·Journal of Periodontal Research·P S Cerri, E Katchburian
Nov 19, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Husheem MichaelTeuvo A Hentunen
Jul 11, 2006·Journal of Anatomy·Estela Sasso-CerriSandra Maria Miraglia
Feb 28, 2007·Trends in Endocrinology and Metabolism : TEM·James D Yager, Jin Q Chen
Apr 5, 2007·Journal of Cellular Biochemistry·Paula H Stern
Apr 25, 2007·Journal of Periodontal Research·A P S FaloniP S Cerri
Jun 6, 2007·Journal of Bioenergetics and Biomembranes·Vladimir Gogvadze, Boris Zhivotovsky
Jun 3, 2008·Archives of Medical Research·Gregor M BranUlrich R Goessler
Jun 24, 2008·Molecular and Cellular Endocrinology·James W SimpkinsVirginia Pearce

❮ Previous
Next ❯

Citations

Nov 20, 2009·Reproductive Biology and Endocrinology : RB&E·Estela Sasso-Cerri
Nov 19, 2011·Journal of Anatomy·Ana Paula de Souza FaloniPaulo Sérgio Cerri
Aug 1, 2015·Journal of Anatomy·José Paulo de Pizzol JúniorPaulo Sérgio Cerri
Jun 8, 2013·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Camil Castelo-Branco, Iris Soveral
Aug 8, 2015·BioMed Research International·Rinaldo Florencio-SilvaPaulo Sérgio Cerri
Mar 8, 2014·Molecular Nutrition & Food Research·Don-Gil LeeTae-Hoo Yi
Apr 29, 2010·The Journal of Reproduction and Development·Shuying HuoSheng Cui
Nov 6, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John R HawseMalayannan Subramaniam
Sep 17, 2020·Cells·Joseph Lorenzo
Jan 8, 2021·Journal of Molecular Histology·Rinaldo Florencio-SilvaPaulo Sérgio Cerri
Jul 31, 2020·Archives of Oral Biology·Lanzhi Deng, Yongwen Guo
Dec 26, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rinaldo Florencio-SilvaPaulo S Cerri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis