PMID: 15362970Sep 15, 2004Paper

Immunohistochemical detection of GHRH and its receptor splice variant 1 in primary human breast cancers

European Journal of Endocrinology
I ChatzistamouHelen Koutselini

Abstract

GHRH is secreted by the hypothalamus and, upon binding to specific GHRH receptors in the pituitary, stimulates growth hormone (GH) production and release from the pituitary. In addition to this neuroendocrine action, accumulated evidence implies additional roles for GHRH in carcinogenesis in non-pituitary tissues. In vitro and in vivo studies have shown that splice variant 1 (SV1) of the GHRH receptor, which is widely expressed in non-pituitary tissues and cancers, can mediate the proliferative effects of GHRH. The aim of the present study was to investigate the operation of an autocrine stimulatory loop between GHRH and SV1 in primary breast tumors. Fifty-three primary breast tumors were evaluated for GHRH and SV1 expression. Expression of GHRH and SV1 was assessed by immunohistochemistry using anti-GHRH SV95 and anti-SV1 2317/5 polyclonal antibodies. About 40% of the specimens tested express GHRH and/or SV1 (approx. 25% each), while in 35% of these positive specimens co-expression of these antigens was detected (P<0.01). Furthermore, a correlation of GHRH, but not SV1, expression was detected in lobular compared with ductal carcinomas. These results constitute the first demonstration for the expression of GHRH and SV1 in prim...Continue Reading

Citations

Feb 7, 2008·Journal of Mammary Gland Biology and Neoplasia·Jo K PerryPeter E Lobie
Dec 18, 2007·Nature Clinical Practice. Endocrinology & Metabolism·Andrew V SchallyJörg B Engel
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zoltan RekasiPeter Nemeth
Jan 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Stefan BuchholzGabor Halmos
Feb 8, 2008·Regulatory Peptides·Ioulia ChatzistamouChristos Kittas
Aug 15, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Solveig SchulzStefan Schulz
Jan 13, 2015·International Journal of Endocrinology·Kerry L Hull, Steve Harvey
Aug 7, 2007·International Journal of Hematology·Brett HallFrank Marini
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Dec 6, 2017·Applied Immunohistochemistry & Molecular Morphology : AIMM·Mahsa KhanlariMehrdad Nadji
Jul 10, 2021·Molecular and Cellular Endocrinology·Yurong Zheng, Antoine E Karnoub
Oct 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Zhaotong CongMing-Wei Wang

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