Immunohistochemical detection of MMP-2 and MMP-9 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation.

International Journal of Legal Medicine
Mizuho NosakaToshikazu Kondo

Abstract

We immunohistochemically examined the expression of matrix metalloproteinase (MMP)-2 and MMP-9 using venous thrombi developed by ligation of the inferior vena cava (IVC) in mice. Both MMP-2- and MMP-9- positive cells could be detected in the whole course of thrombus formation after IVC ligation. Morphometrically, their number was greatest 14 days after IVC ligation and thereafter, gradually decreased at 21 days. The number of MMP-9-positive cells was significantly higher than that of MMP-2-positive cells at 1 to 7 days. The average ratio of MMP-9 to MMP-2 (MMP-9/MMP-2 ratio) was >2.0 in all thrombus samples at 1-5 days. After 7 days, the MMP-9/MMP-2 ratio was less than 2.0. These observations implied that an MMP-9/MMP-2 ratio markedly exceeding 2.0 strongly indicates an age of 5 days or less. Furthermore, an MMP-9/MMP-2 ratio of <2.0 probably indicates an age of more than 7 days. The present study demonstrated that the immunohistochemical detection of intrathrombotic MMP-2 and MMP-9 was suitable to estimate the age of venous thrombi.

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Citations

Feb 1, 2012·Der Pathologe·T BajanowskiR Dettmeyer
Feb 4, 2012·International Journal of Legal Medicine·Rossana CecchiStefano Ferracuti
Mar 9, 2012·International Journal of Legal Medicine·Yuko IshidaToshikazu Kondo
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Mar 11, 2015·International Journal of Legal Medicine·Yuko IshidaToshikazu Kondo
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Oct 21, 2017·International Journal of Legal Medicine·Valeria MaffeisAmbrogio Fassina
Oct 26, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Mizuho NosakaToshikazu Kondo
Oct 31, 2017·International Journal of Legal Medicine·Yuko IshidaToshikazu Kondo
Nov 13, 2019·International Journal of Legal Medicine·Mizuho NosakaToshikazu Kondo
Mar 22, 2020·International Journal of Molecular Sciences·J Matthew NicklasPeter K Henke
Jan 8, 2021·International Journal of Legal Medicine·Mizuho NosakaToshikazu Kondo
Dec 23, 2020·International Journal of Legal Medicine·P MarkwerthR Dettmeyer

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