Immunohistochemical detection of P-glycoprotein (clone C494) in canine mammary gland tumours

Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine
C PetterinoM Castagnaro

Abstract

Elevated levels of P-glycoprotein have been reported in multidrug-resistant tumours in both humans and dogs. In the present study, we investigated the expression of P-glycoprotein in 57 canine mammary gland tumours, 10 mammary gland hyperplasia and seven normal mammary glands by immunohistochemistry. Tissue sections were incubated with an anti-Pgp monoclonal antibody and visualized with En Vision-DAB polymer. Normal and hyperplastic mammary tissues were negative or showed slight cytoplasmic immunoreactivity. Neoplastic cells in benign mammary tumours showed diffuse cytoplasmic staining, in contrast to malignant tumours that showed mainly a membranous staining pattern for Pgp (C494). We observed statistically significant differences among all the different groups of tissues analysed except for benign tumours versus hyperplasia (P = 0.221). Receiver-operating characteristic analysis showed that the best cut-off point to differentiate the threshold to differentiate negative from positive tissue samples was 18.40% of immunostained cells. These results provide a first indication that routine evaluation of Pgp expression in canine mammary gland tumours, taking into consideration a cut-off point for positivity, may be useful for selec...Continue Reading

References

Dec 5, 1991·The New England Journal of Medicine·H S ChanV Ling
Jan 1, 1990·Human Pathology·R S WeinsteinJ S Coon
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F ThiebautM C Willingham
Jul 14, 1989·Biochemical and Biophysical Research Communications·D M LiebermanK L Skorecki
Dec 1, 1989·British Journal of Cancer·J SchneiderM Volm
Jun 3, 1988·Science·J A Swets
Nov 23, 1995·The New England Journal of Medicine·N BaldiniM Campanacci
Jan 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A G van der ZeeE G de Vries
Apr 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S C LinnG Giaccone
Nov 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A R Yuen, B I Sikic
Sep 1, 1995·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S C LinnJ P Baak
Jun 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·U A Germann
May 29, 1997·International Journal of Cancer. Journal International Du Cancer·S C LinnG Giaccone
May 20, 1999·Annual Review of Pharmacology and Toxicology·S V AmbudkarM M Gottesman
Dec 7, 2000·Cancer Investigation·D M Vail, E G MacEwen
Dec 1, 2004·Veterinary and Comparative Oncology·M D LucroyR A LeCouteur

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Citations

Oct 1, 2010·Journal of Comparative Pathology·S Van der HeydenR Ducatelle
Sep 17, 2009·Reproduction in Domestic Animals = Zuchthygiene·K U HonschaG Gäbel
Mar 25, 2014·Acta Veterinaria Hungarica·Zsófia Koltai, Péter Vajdovich
Mar 15, 2021·Journal of Comparative Pathology·Verônica Correia ManoelBruno Cogliati

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