Immunohistochemical detection of telomerase (hTERT) protein in human cancer tissues and a subset of cells in normal tissues

Neoplasia : an International Journal for Oncology Research
E HiyamaJ W Shay

Abstract

We examined human telomerase reverse transcriptase (hTERT) protein distribution by immunohistochemistry in cultured cells and tissue sections. Cells with telomerase activity had nuclear positive signals whereas cells without telomerase activity did not. In most normal epithelial tissues, hTERT expression was prominent in the early proliferative descendent progenitors cells. In cancers with high telomerase activity, hTERT expression was detected in almost all neoplastic cells and correlated with telomerase activity levels, whereas cancers with low telomerase activity had fewer hTERT-positive cancer cells. In pediatric neuroblastomas with a favorable outcome, both the percentage of positive cells and the signal intensities of each hTERT-expressing cell decreased. These studies indicate that detection of telomerase at the cellular level is achievable and may have utility in cancer diagnostics.

Citations

Mar 29, 2014·International Journal of Molecular Medicine·Mariann Friis-OttessenOle Petter F Clausen
Nov 13, 2008·Cytotechnology·Eiso Hiyama, Keiko Hiyama
May 27, 2009·Indian Journal of Urology : IJU : Journal of the Urological Society of India·Sara BravacciniRosella Silvestrini
Nov 19, 2010·Molecular Biology Reports·Aneta WojtylaBlazej Rubis
Feb 19, 2002·Oncogene·Eiso Hiyama, Keiko Hiyama
Mar 5, 2005·Reproduction, Fertility, and Development·S Schaetzlein, K L Rudolph
Jun 21, 2006·Applied Immunohistochemistry & Molecular Morphology : AIMM·Muna SabahElaine Kay
Jun 22, 2007·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Baisakhi SahaS Ashraf Imam
Oct 26, 2007·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Yasushi HashimotoEiso Hiyama
Oct 30, 2008·Ultrastructural Pathology·Sebastien LepreuxMarie-Laure Martin-Negrier
Aug 3, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeanne GalaineOlivier Adotévi
Jan 19, 2019·Cells·Petra Procházková SchrumpfováJiří Fajkus
Jul 13, 2002·Expert Review of Anticancer Therapy·L Austin Doyle, W Edward Highsmith
Aug 24, 2004·Advances in Anatomic Pathology·Atac BaykalAysegul A Sahin
Apr 10, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ching-Hsiu YangChee-Yin Chai
Aug 23, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Serena PanareseGiuseppe Sarli
Dec 11, 2013·Current Opinion in Pediatrics·Piotr CzaudernaRebecka L Meyers
Jan 17, 2020·Journal of Oral and Maxillofacial Pathology : JOMFP·Namrata MishraVijay Kumar
Apr 12, 2002·International Journal of Cancer. Journal International Du Cancer·Pu YanLouis Guillou
Aug 2, 2003·In Vitro Cellular & Developmental Biology. Animal·Robert L OchsPaul Kornblith
Dec 23, 2004·Histochemistry and Cell Biology·Esther Asan, Detlev Drenckhahn
Sep 10, 2005·Expert Review of Molecular Diagnostics·John P Jakupciak
Feb 15, 2011·The International Journal of Biological Markers·Mahendar PorikaSadanandam Abbagani
May 10, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gulden DinizCanan Vergin
Aug 8, 2014·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Yaojian RaoJiawei Cui
Jun 4, 2016·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Su-Yang LiuGregor Krings
Dec 22, 2017·Oncotarget·Jaskaren S KohliDorothy C Bennett
May 15, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Joern KamradtBernd Wullich
Feb 19, 2002·Oncogene·Kathleen Collins, James R Mitchell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.