Abstract
We employed the polyclonal anti-p53 antibody NCL-CM1 to cultured cells and pathological tissues in order to investigate the expression of p53 oncoprotein in human malignant melanomas. The results in the cultured cells showed that the antigenic determinant was sensitive to formalin fixation, resulting in a lower reactivity than with fixation by alcohol. In pathological tissues, the expression of p53 oncoprotein increased with progression of the tumour. Among 79 melanomas 37 (47%) showed distinct nuclear labelling and the highest proportion of reactive cells was observed in metastatic melanomas (mean 4.8%). An immunocytochemical study also revealed the presence of mutant-type oncoprotein in human melanoma cell lines, which was recognized by monoclonal antibody P240, and we confirmed that the molecular weight of the antigens recognized by both antibodies was 53 kDa by Western blot analysis. Therefore, although the presence of point mutations in human melanomas is yet to be confirmed our data suggest that the antigen detected by NCL-CM1 is a mutant-type or a complex of mutant and wild-type p53 oncoproteins. This antibody may be useful in retrospective studies of tumours of melanocytic origin.
References
Mar 15, 1979·Nature·D P Lane, L V Crawford
Apr 1, 1979·Pathology·J H Pope Regius Mary Sister
Apr 1, 1992·The Journal of Pathology·M BarbareschiC Doglioni
Mar 1, 1992·The Journal of Pathology·R VilluendasP Martinez
Jan 1, 1992·Melanoma Research·H TakahashiP G Parsons
Aug 19, 1992·International Journal of Cancer. Journal International Du Cancer·L A Akslen, O Mørkve
Jun 1, 1992·The American Journal of Dermatopathology·C S SimP H McKee
Apr 1, 1992·The Journal of Pathology·D Wynford-Thomas
Jan 1, 1992·Japanese Journal of Cancer Research : Gann·H HiyoshiS Hirohashi
Mar 1, 1992·Histopathology·S D SlaterP H McKee
Mar 1, 1991·Histopathology·H TakahashiP G Parsons
Jul 1, 1991·Japanese Journal of Cancer Research : Gann·K IwayaS Hirohashi
Jan 1, 1991·Archives of Dermatological Research·K PerisH Höfler
Nov 1, 1990·Pigment Cell Research·H Takahashi, P G Parsons
Nov 1, 1991·The Journal of Pathology·D Wynford-Thomas
Dec 1, 1991·British Journal of Cancer·C M BartonR C Williamson
Feb 1, 1991·Pigment Cell Research·M VolkenandtA P Albino
Mar 24, 1990·Lancet·R IggoA L Harris
Apr 4, 1991·Nature·A L Harris
May 1, 1991·The Journal of Pathology·J L OstrowskiC H Horne
Feb 1, 1988·Molecular and Cellular Biology·C A FinlayA J Levine
Citations
Jan 1, 1995·Archives of Dermatological Research·M YamamotoM Takahashi
Jun 1, 1996·Journal of Dermatological Science·M KanokoM Ichihashi
Jul 3, 1998·The American Journal of Dermatopathology·K J SmithH M Skelton
Dec 1, 1993·Journal of Cutaneous Pathology·M R WickJ F Fitzgibbon
Dec 1, 1995·Journal of Cutaneous Pathology·N S McNuttJ Lugo
Aug 8, 1998·Journal of Cutaneous Pathology·C MiraccoP Luzi
Feb 1, 1995·Experimental Dermatology·S InoharaK Kitagawa
Nov 24, 2004·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mahmoud R Hussein
Aug 1, 1996·Pathology, Research and Practice·L TalveT Ekfors
Sep 24, 2004·British Journal of Plastic Surgery·David ChinWilliam B Coman
Nov 1, 1996·Pathology·V JayM Zielenska
Mar 13, 2014·Journal of Clinical Pathology·Johanne Lade-KellerTorben Steiniche
Feb 24, 2001·The Laryngoscope·S EdströmC Young
Jul 29, 1996·International Journal of Cancer. Journal International Du Cancer·A HartmannJ S Kovach
Sep 26, 2009·Neurologia Medico-chirurgica·Kazuki MasuMakoto Sasaki
Aug 2, 2003·Oncogene·Julia V KichinaAndrei V Gudkov
Aug 15, 1994·International Journal of Cancer. Journal International Du Cancer·K AngelopoulouP S Bunting
Jun 29, 2005·Molekuliarnaia biologiia·V I TarasenkoIu M Konstantinov
Jul 1, 1995·The British Journal of Dermatology·J WeissE G Jung