Immunohistochemical detection of uPA, tPA, and PAI-1 in a stasis-induced deep vein thrombosis model and its application to thrombus age estimation.

International Journal of Legal Medicine
Mizuho NosakaToshikazu Kondo

Abstract

We immunohistochemically examined the expression of urokinase-type plasminogen activator (uPA), tissue-type plasminogen activator (tPA), and plasminogen-activator inhibitor type-1 (PAI-1) using venous thrombi developed by ligation of the inferior vena cava (IVC) in mice. The uPA-, tPA- and PAI-1-positive cells could be firstly detected 5, 7, and 3 days, respectively, after IVC ligation. Morphometrically, the number of PAI-1-positive cells was significantly higher than those of uPA- and tPA-positive cells at later than 7 days. In all of the thrombus samples aged 10-21 days, the uPA/PAI-1 and tPA/PAI-1 ratios were >0.1 and >0.2, respectively. In contrast, all of the thrombus samples aged 1-7 days had uPA/PAI-1 of <0.1 and tPA/PAI-1 ratios of <0.2. These findings implied that uPA/PAI-1 of >0.1 and tPA/PAI-1 of >0.2 indicated an age of 10 days or more. Moreover, in four of five samples aged 10 days, uPA/PAI-1 ratios were <0.3, and the remaining one had uPA/PAI-1 of 0.32. All thrombi aged 14-21 days showed values greater than 0.3. Thus, uPA/PAI-1 ratios, markedly exceeding 0.3, strongly indicated an age of more than 14 days. The present study demonstrated that the immunohistochemical detection of uPA, tPA, and PAI-1 was suitable to ...Continue Reading

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Citations

Feb 1, 2014·Legal Medicine·Hitoshi MaedaTomomi Michiue
Jan 27, 2015·International Journal of Legal Medicine·Mizuho NosakaToshikazu Kondo
Oct 21, 2017·International Journal of Legal Medicine·Valeria MaffeisAmbrogio Fassina
Oct 26, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Mizuho NosakaToshikazu Kondo
Nov 13, 2019·International Journal of Legal Medicine·Mizuho NosakaToshikazu Kondo
Dec 23, 2020·International Journal of Legal Medicine·P MarkwerthR Dettmeyer

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