Immunohistochemical detection of vascular endothelial growth factor (VEGF) and VEGF receptors Flt-1 and KDR/Flk-1 in the cochlea of guinea pigs

Hearing Research
O MichelK Addicks

Abstract

Vascular endothelial growth factor (VEGF) is known as an endothelial cell-specific mitogen. There are no reports concerning the presence of VEGF in the inner ear. To gain information, immunohistochemical analysis using specific antibodies to VEGF and to both known VEGF receptors Flt-1 and KDR/Flk-1 was performed on paraffin-sectioned temporal bones from five guinea pigs. Immunoreactivity of VEGF, Flt-1 and KDR/Flk-1 was detectable in spiral ganglion cells. VEGF could also be found in the endothelium of blood vessels, in the spiral ligament and in the organ of Corti. Flt-1 was found in the limbus epithelium, in all supporting cells of the organ of Corti, in Claudius cells, cells of the sulcus and in the spiral ligament. Flk-1 could be detected in some supporting cells of the organ of Corti (inner pillar cells and Deiters' cells). Immunoreactivity to Flk-1 was also found in endothelium of blood vessels and in the spiral ligament. Hair cells showed VEGF immunostaining, but did not contain staining to Flt-1 nor Flk-1. In the stria vascularis any immunoreactivity to all used VEGF and VEGF receptor antibodies could not be detected. The findings were supported by Western blot analysis on inner ear tissues and ovaries from guinea pigs....Continue Reading

References

Feb 17, 1998·Cardiovascular Research·B MalavaudJ Plouët
Dec 5, 1998·Biochemical and Biophysical Research Communications·J Kroll, J Waltenberger
Jul 23, 1999·Hearing Research·O MichelK Addicks

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Citations

Sep 19, 2003·Hearing Research·Johann GrossT Kietzmann
Aug 4, 2004·Experimental Gerontology·Pasqualina PicciottiRoberto Pola
Mar 6, 2012·Journal of Neurogenetics·Mariko HiramatsuTsutomu Nakashima
Feb 22, 2012·Medical Hypotheses·Emilio Carlo CamposAntonio Pirodda
Oct 13, 2006·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Oxana SelivanovaWolf Mann
Dec 3, 2009·International Journal of Audiology·Anna Rita FetoniDiana Troiani
Sep 19, 2009·Cellular and Molecular Life Sciences : CMLS·Arianne Monge NaldiDaniel Bodmer

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