Immunohistochemical detection of ZAP70 in chronic lymphocytic leukemia predicts immunoglobulin heavy chain gene mutation status and time to progression.

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Joan H AdmirandLynne V Abruzzo

Abstract

Zeta-associated protein-70 (ZAP70) expression measured by flow cytometry has been proposed as a surrogate marker of the somatic mutation status of the immunoglobulin heavy chain variable region (IGHV) genes in chronic lymphocytic leukemia. However, attempts to implement this approach in clinical flow cytometry laboratories have been problematic; many commercially available antibodies give unreliable results. Assessment of ZAP70 protein expression by immunohistochemistry in chronic lymphocytic leukemia tissue sections is an easy, alternative approach, although lack of quantitation and subjective interpretation of results are potential pitfalls. In this study, we correlated ZAP70 protein expression, assessed by immunohistochemistry, with ZAP70 messenger RNA (mRNA) transcript expression, assessed by semi-quantitative real-time reverse transcriptase-polymerase chain reaction assay, with the somatic mutation status of the IGHV genes in previously untreated patients with chronic lymphocytic leukemia. Expression of ZAP70 protein and mRNA transcripts correlated strongly (P=8.238 × 10(-12)). Expression of ZAP70 protein and mRNA transcripts also correlated strongly with the somatic mutation status of the IGHV genes (P=0.000071 and P=0.00...Continue Reading

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Citations

Feb 24, 2018·Current Hematologic Malignancy Reports·Prajwal Boddu, Alessandra Ferrajoli
Mar 1, 2012·Expert Review of Anticancer Therapy·Ali BazarganMichael J Keating
Jun 3, 2020·Journal of the American Medical Informatics Association : JAMIA·Caitlin E CoombesKevin R Coombes
Apr 17, 2021·Journal of Biomedical Informatics·Caitlin E CoombesGuy Brock

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Methods Mentioned

BETA
flow cytometry
biopsies
PCR
Assay
flow-cytometry

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