Immunohistochemical determination of calcium-calmodulin binding predicts neuronal damage after global ischemia

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
C M PiconeJ R Dedman

Abstract

Since ionic Ca2+ binds with intracellular calmodulin (CaM) before activating proteases, kinases, and phospholipases, demonstration of persistent Ca2+-CaM binding in neurons destined to show ischemic cellular injury would support the concept that elevated intracellular Ca2+ plays a causative role in ischemic neuronal damage. In order to characterize Ca2+-CaM binding, we used a sheep anti-CaM antibody (CaM-Ab) which recognizes CaM that is not bound to Ca2+ or brain target proteins. Therefore, immunohistochemical staining of brain sections by labeled CaM-Ab represented only unbound CaM. Six normal rats were compared to 15 animals rendered ischemic for 30 min by a modification of the four-vessel occlusion model. Animals were killed immediately after ischemia, and after 2 and 24 h of reperfusion. Brain sections through hippocampus were incubated in CaM-Ab, and a diaminobenzadiene labeled anti-sheep secondary antibody was added to stain the CaM-Ab. Staining in the endal limb of dentate, dorsal CA1, lateral CA3, and parietal cortex was graded on a 4-point scale. All normal animals had grade 4 staining indicating the presence of unbound CaM in all four brain regions. Ischemic animals demonstrated reduced (grade 0 to 2) staining in the ...Continue Reading

References

Aug 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·E MartinsB K Siesjö
Apr 1, 1988·Stroke; a Journal of Cerebral Circulation·J C GrottaD McCandless
Oct 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H Benveniste, N H Diemer
Aug 5, 1987·Neuroscience Letters·E DuxL Siklós
Feb 1, 1987·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J K DeshpandeT Wieloch
Mar 1, 1986·Stroke; a Journal of Cerebral Circulation·J GrottaD Hunter
Mar 1, 1985·Stroke; a Journal of Cerebral Circulation·J GrottaH Gary
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C C OuimetP Greengard
Jan 1, 1983·Methods in Enzymology·J R Dedman, M A Kaetzel
Sep 1, 1983·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K A HossmannL Csiba
May 1, 1982·Annals of Neurology·W A PulsinelliF Plum

❮ Previous
Next ❯

Citations

Jan 1, 1991·Neuroscience·R Schmidt-Kastner, T F Freund
Feb 12, 1998·European Journal of Pharmacology·M SugimuraY Shirasaki
May 13, 1999·Progress in Neurobiology·M MoriokaE Miyamoto
Oct 20, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A MitaniK Kataoka
Nov 2, 2004·Biological & Pharmaceutical Bulletin·Hiromichi TakanoYasufumi Shirasaki
Apr 2, 2005·Biological & Pharmaceutical Bulletin·Masunobu SugimuraYasufumi Shirasaki
Mar 1, 1990·The Histochemical Journal·M BorgersJ Van Reempts
Sep 3, 2004·Brain Research·Yasufumi ShirasakiToshiyuki Sato
Mar 21, 2006·Brain Research·Yasufumi ShirasakiMitsuhiro Makino
Oct 1, 2005·Techniques in Vascular and Interventional Radiology·Venkatakrishna Rajajee, Jeffrey Saver
Sep 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S TakizawaA M Hakim
Jul 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J AronowskiJ C Grotta
Mar 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T J DeGrabaJ C Grotta
Mar 1, 1994·Stroke; a Journal of Cerebral Circulation·D GeorgiadisK R Lees
Feb 1, 1994·Stroke; a Journal of Cerebral Circulation·S K HansonP Ostrow
Jun 1, 1990·Stroke; a Journal of Cerebral Circulation·J C GrottaJ R Dedman
Jun 1, 1992·Stroke; a Journal of Cerebral Circulation·T J DeGrabaJ C Grotta
Nov 28, 1993·Biochimica Et Biophysica Acta·F A SchanneP K Stanton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.