Immunohistochemical diagnosis of mucous membrane pemphigoid

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Iakov ShimanovichChristian Rose

Abstract

Mucous membrane pemphigoid (MMP) is an autoimmune subepithelial blistering disease with predominant involvement of mucosal surfaces. It is usually diagnosed by direct immunofluorescence microscopy of frozen biopsies, demonstrating linear deposits of complement, IgG or IgA along the basement membrane. The aim of this study was to investigate the utility of immunohistochemistry on formalin-fixed, paraffin-embedded tissue biopsies for the diagnosis of MMP and to compare its sensitivity to that of direct immunofluorescence microscopy. We examined 50 biopsies from 34 patients with immunologically confirmed MMP by immunohistochemistry for C3d, C4d, IgG and IgA. Linear deposits of C3d were detected in 46% of biopsies, and 53% of patients had at least one biopsy positive for C3d. Linear deposits of C4d were detected in 52% of biopsies and 59% of patients had at least one biopsy positive for C4d. Overall, 56% of biopsies and 68% of patients were positive by either C3d or C4d or both stainings. The sensitivity of either staining in mucosal biopsies was lower than in skin samples. Basement membrane deposits of IgG or IgA could not be detected in any biopsy. Our findings demonstrate that immunohistochemistry for C3d or C4d is a helpful scr...Continue Reading

References

Oct 31, 2012·Archives of Pathology & Laboratory Medicine·Xuefeng ZhangChristopher R Shea
Dec 3, 2014·Journal of the American Academy of Dermatology·Dedee F MurrellVictoria P Werth
Jul 6, 2017·Journal of the American Academy of Dermatology·Iakov ShimanovichDetlef Zillikens

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Citations

Dec 27, 2018·The British Journal of Dermatology·S SunX Zeng
Jun 17, 2020·Der Pathologe·Nina van BeekDetlef Zillikens
Feb 12, 2019·Frontiers in Immunology·Mayumi Kamaguchi, Hiroaki Iwata
Apr 25, 2021·Letters in Applied Microbiology·Jing SunLiangpeng Ge

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